尼日利亚阿布贾教学医院收治的人类免疫缺陷病毒与利什曼病合并感染患者的白细胞及Th相关细胞因子谱
Leucocytes and Th-associated Cytokine Profile of HIV-Leishmaniasis Co-Infected Persons Attending Abuja Teaching Hospital, Nigeria.
作者信息
Abdullahi Idris Nasir, Emeribe Anthony Uchenna, Adekola Hafeez Aderinsayo, Muhammad Habiba Yahaya, Ahmad Abdurrahman El-Fulaty, Anka Abubakar Umar, Mohammed Yusuf, Haruna Shamsuddeen, Oderinde Bamidele Soji, Shuwa Halima Ali, Babayo Adamu
机构信息
Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Ahmadu Bello University, Zaria, Nigeria.
Department of Medical Laboratory Science, University of Calabar, Calabar, Nigeria.
出版信息
Eurasian J Med. 2020 Oct;52(3):271-276. doi: 10.5152/eurasianjmed.2020.20008.
OBJECTIVE
T-helper cells (Th)-1& -2 cytokines homeostasis control or predict clinical outcome of infected persons, especially those with HIV /AIDS. This case-control study evaluated the leucocytes differentials, TNF-alpha, interleukin (IL)-2 and -10 levels among HIV infected persons with serological evidence of leishmaniasis attending University of Abuja Teaching Hospital, Nigeria.
MATERIALS AND METHODS
Blood samples from 28 HIV infected persons who had Leishmania donovani rK39 and Immunoglobulin-G (IgG) positive (group 1), 30 age- & -sex matched HIV infected persons without Leishmania antibodies (group 2) and 30 apparently healthy persons without HIV and Leishmania antibodies (group 3). Full blood counts, TNF alpha, IL-2 and -10 levels were analyzed using automated hematology analyzer and ELISA, respectively. Structured questionnaires were used to collate biodata and clinical presentations of participants.
RESULTS
Ten (35.7%) participants in group 1 were on ART, 15 (50%) in group 2 were on ART, while group 3 were ART naïve. There were significantly higher values in basophil (4.4±2.5%) and eosinophil counts (12.9±3.8%) in HIV/leishmania coinfected persons (p<0.005). However, other white cells subpopulation was significantly lower in HIV/leishmania co-infected participants (p<0.05). There was significantly reduced CD4+ T cell counts ([119±26 versus 348±63 versus 605±116 cells/mm3]), TNF-alpha ([36.82±8.21 versus 64.67±12.54 versus 254.98±65.59 pg/mL]) and IL-2 levels ([142.14±20.91 versus 507.6±84.42 versus 486.62±167.87 pg/mL]) among HIV/Leishmania co-infected participants compared to group 2 and group 3 participants, respectively. However, higher IL-10 level (80.35±14.57 pg/mL) was found in HIV/Leishmania co-infected participants as opposed to the HIV monoinfected (62.2±10.43 pg/mL) and apparently healthy persons (23.97±4.88 pg/mL) (p<0.001).
CONCLUSION
Eosinophil, basophil counts and serum IL-10 level were high in HIV/Leishmania coinfected persons, demonstrating parasite-induced hypersensitivity and immunosuppression.
目的
辅助性T细胞（Th）-1和-2细胞因子的稳态控制或可预测感染者尤其是艾滋病毒/艾滋病患者的临床结局。本病例对照研究评估了在尼日利亚阿布贾大学教学医院就诊的、有利什曼病血清学证据的艾滋病毒感染者的白细胞分类、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-2和-10水平。
材料与方法
采集28例杜氏利什曼原虫rK39和免疫球蛋白-G（IgG）呈阳性的艾滋病毒感染者（第1组）、30例年龄和性别匹配的无利什曼原虫抗体的艾滋病毒感染者（第2组）以及30例无艾滋病毒和利什曼原虫抗体的明显健康者（第3组）的血样。分别使用自动血液分析仪和酶联免疫吸附测定法（ELISA）分析全血细胞计数、TNF-α、IL-2和-10水平。使用结构化问卷整理参与者的生物数据和临床表现。
结果
第1组中有10例（35.7%）参与者接受抗逆转录病毒治疗（ART），第2组中有15例（50%）接受ART，而第3组未接受过ART。艾滋病毒/利什曼病合并感染的患者嗜碱性粒细胞（4.4±2.5%）和嗜酸性粒细胞计数（12.9±3.8%）显著更高（p<0.005）。然而，艾滋病毒/利什曼病合并感染的参与者中其他白细胞亚群显著更低（p<0.05）。与第2组和第3组参与者相比，艾滋病毒/利什曼病合并感染的参与者中CD4+T细胞计数（[分别为每立方毫米119±26个对348±63个对605±116个细胞]）、TNF-α（[分别为36.82±8.21对64.67±12.54对254.98±65.59皮克/毫升]）和IL-2水平（[分别为142.14±20.91对507.6±84.42对486.62±167.87皮克/毫升]）显著降低。然而，与艾滋病毒单一感染（62.2±10.43皮克/毫升）和明显健康者（23.97±4.88皮克/毫升）相比，艾滋病毒/利什曼病合并感染的参与者中IL-10水平更高（80.35±14.57皮克/毫升）（p<0.001）。
结论
艾滋病毒/利什曼病合并感染的患者嗜酸性粒细胞、嗜碱性粒细胞计数和血清IL-10水平较高，表明寄生虫诱导的超敏反应和免疫抑制。
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