Parks L W, Bond F T, Thompson E D, Starr P R
J Lipid Res. 1972 May;13(3):311-6.
Whereas wild-type strains of Saccharomyces cerevisiae can synthesize up to 7% dry weight of ergosterol, a polyene-resistant mutant has been obtained which produces no ergosterol. Instead, a C-28 methyl sterol is produced, and it has been identified as Delta(8(9),22)-ergostadiene-3beta-ol. This sterol is converted to ergosterol by wild-type yeasts and is observed transiently in cells during aerobic adaption of anaerobically grown wild-type yeasts. The new sterol is proposed as an intermediate in ergosterol biosynthesis.
酿酒酵母的野生型菌株能够合成高达干重7%的麦角固醇，而现已获得一种对多烯耐药的突变体，该突变体不产生麦角固醇。相反，它产生一种C - 28甲基固醇，已鉴定为Δ(8(9),22)-麦角二烯-3β-醇。这种固醇可被野生型酵母转化为麦角固醇，并且在厌氧生长的野生型酵母进行需氧适应过程中，可在细胞中短暂观察到。新的固醇被认为是麦角固醇生物合成的一种中间体。
