Department of Microbiology, Universität Innsbruck, Technikerstraße 25d, 6020, Innsbruck, Austria.
Department of Zoology, Universität Innsbruck, Technikerstraße 25, 6020, Innsbruck, Austria.
Antonie Van Leeuwenhoek. 2021 Jan;114(1):45-54. doi: 10.1007/s10482-020-01497-0. Epub 2020 Nov 19.
We isolated a filamentous, thermophilic, and first anaerobic representative of the genus Thermoactinomyces, designated strain AMNI-1, from a biogas plant in Tyrol, Austria and report the results of a phenotypic, genetic, and phylogenetic investigation. Strain AMNI-1 was observed to form a white branching mycelium that aggregates into pellets when grown in liquid medium. Cells could primarily utilize lactose, glucose, and mannose as carbon and energy sources, with acetate accelerating and yeast extract being mandatory for growth. The optimum growth temperature and pH turned out to be 55 °C and pH 7.0, respectively, with an optimum NaCl concentration of 0-2% (w/v). 16S rRNA gene sequence comparison indicated that the genetic relatedness between strain AMNI-1 and Thermoactinomyces intermedius, Thermoactinomyces khenchelensis, and Thermoactinomyces vulgaris was less than 97%. The G + C content of the genomic DNA was 44.7 mol%. The data obtained suggest that the isolate represents a novel and first anaerobic species of the genus Thermoactinomyces, for which the name Thermoactinomyces mirandus is proposed. The type strain is AMNI-1 (= DSM 110094 = LMG 31503). The description of the genus Thermoactinomyces is emended accordingly.
我们从奥地利蒂罗尔的一个沼气厂中分离出了一种丝状、嗜热且为首次发现的厌氧型嗜热放线菌属代表菌株，命名为 AMNI-1 株，并对其进行了表型、遗传和系统发育研究。观察到 AMNI-1 株形成白色分支状菌丝体，在液体培养基中生长时会聚集形成颗粒。细胞主要利用乳糖、葡萄糖和甘露糖作为碳源和能源，而乙酸盐可加速生长，酵母提取物是生长必需的。最佳生长温度和 pH 值分别为 55°C 和 pH 7.0，最佳 NaCl 浓度为 0-2%（w/v）。16S rRNA 基因序列比较表明，菌株 AMNI-1 与中间型嗜热放线菌、嗜热解糖放线菌和普通嗜热放线菌的遗传关系小于 97%。基因组 DNA 的 G+C 含量为 44.7 mol%。获得的数据表明，该分离株代表了嗜热放线菌属中的一种新型且首次发现的厌氧种，建议将其命名为温和嗜热放线菌。模式菌株为 AMNI-1（=DSM 110094=LMG 31503）。相应地对嗜热放线菌属的描述进行了修正。
