Zhang Q, Len T-Y, Zhang S-X, Zhao Q-H, Yang L-H
Department of Gynaecology, The Second Affiliated Hospital of Kunming Medical University, Kunming, P.R. China.
Eur Rev Med Pharmacol Sci. 2020 Nov;24(21):10921. doi: 10.26355/eurrev_202011_23560.
The article "Exosomes transferring long non-coding RNA FAL1 to regulate ovarian cancer metastasis through the PTEN/AKT signaling pathway, by Q. Zhang, T.-Y. Len, S.-X. Zhang, Q.-H. Zhao, L.-H. Yang, published in Eur Rev Med Pharmacol Sci 2020; 24 (1): 43-54-DOI: 10.26355/eurrev_202001_19894-PMID: 31957817" has been withdrawn from the authors stating that "after the manuscript has been accepted, we are ready to continue to study the exosomes and their mechanism of action. Before the research, we read the latest guideline of exosomes research, MISEV2018. This guideline first suggests that extracellular vesicles should be used to refer to these cell-derived noncellular membrane structures, while exosomes are only applicable to those vesicles released from intracellular sources to extracellular cells by special means. Secondly, the guidelines suggest that when performing key functional verification experiments with extracellular vesicles, methods such as density gradient centrifugation should be used to purify the vesicles. Thirdly, strict negative control should be set up in the functional study of cells, such as cell-conditioned medium treated with extracellular vesicle production inhibitor (GW4869), so as to exclude the false positive of other non-extracellular vesicle components in functional analysis. In our published manuscripts, we called extracellular vesicles as exosomes, and used exosomes separation kit with low purity to separate the exosomes. No appropriate negative control is used in the functional analysis. Most importantly, the conclusion we made in our study is "SKOV3-secreted exosomes inhibited the PTEN/AKT signaling pathway by transferring lncRNA FAL1, thus inhibiting OC cell metastasis in vitro and in vivo". However, the study did not confirm whether lncRNA FAL1 was encapsulated by extracellular vesicles and transferred to OC cells or induced by extracellular vesicles to upregulate its expression in OC cells. Based on the above reasons, we believe that our understanding of extracellular vesicles is not deep enough, which leads to the inaccuracy and over-interpretation of the experimental results. In order to avoid the readers' misunderstanding of extracellular vesicles and ensure the preciseness of scientific research, all of our authors decided to withdraw this article. We will conduct our research again according to MISEV2018, interpret the experimental results and write articles again, and will submit to ERMPS in the near future". The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/19894.
张Q、Len T - Y、Zhang S - X、Zhao Q - H、Yang L - H发表于《欧洲医学与药理学评论》2020年；24(1):43 - 54 - DOI: 10.26355/eurrev_202001_19894 - PMID: 31957817的文章《外泌体通过PTEN/AKT信号通路转运长链非编码RNA FAL1调控卵巢癌转移》已被作者撤回，作者称：“稿件被接受后，我们准备继续研究外泌体及其作用机制。在研究之前，我们阅读了外泌体研究的最新指南MISEV2018。该指南首先建议，细胞外囊泡应用于指代这些细胞来源的非细胞内膜结构，而外泌体仅适用于那些通过特殊方式从细胞内释放到细胞外的囊泡。其次，该指南建议，在用细胞外囊泡进行关键功能验证实验时，应采用密度梯度离心等方法纯化囊泡。第三，在细胞功能研究中应设置严格的阴性对照，如用细胞外囊泡产生抑制剂（GW4869）处理的细胞条件培养基，以排除功能分析中其他非细胞外囊泡成分的假阳性。在我们已发表的稿件中，我们将细胞外囊泡称为外泌体，并使用低纯度的外泌体分离试剂盒分离外泌体。在功能分析中未使用适当的阴性对照。最重要的是，我们在研究中得出的结论是‘SKOV3分泌的外泌体通过转运lncRNA FAL1抑制PTEN/AKT信号通路，从而在体外和体内抑制OC细胞转移’。然而，该研究并未证实lncRNA FAL1是否被细胞外囊泡包裹并转移至OC细胞，或是否由细胞外囊泡诱导在OC细胞中上调其表达。基于上述原因，我们认为我们对细胞外囊泡的理解不够深入，导致实验结果不准确且过度解读。为避免读者对外泌体产生误解并确保科研的精确性，我们所有作者决定撤回本文。我们将根据MISEV2018重新开展研究，解读实验结果并重新撰写文章，并将在不久后提交给《欧洲医学与药理学评论》。”出版商对由此可能造成的不便表示歉意。https://www.europeanreview.org/article/19894
