Capital University of Economics and Business, Beijing, China.
Beijing Forestry University, Beijing, China.
PLoS One. 2020 Nov 20;15(11):e0242221. doi: 10.1371/journal.pone.0242221. eCollection 2020.
An economically valuable woody plant species tree bean (Cajanus cajan (L.) Millsp.) is predominantly cultivated in tropical and subtropical areas and is regarded as an important food legume (or pulse) crop that is facing serious sodium ion stress. NAM (N-acetyl-5-methoxytryptamine) has been implicated in abiotic and biotic stress tolerance in plants. However, the role of NAM in sodium ion stress tolerance has not been determined. In this study, the effect of NAM was investigated in the economically valuable woody plant species, challenged with stress at 40 mM sodium ion for 3 days. NAM-treated plants (200 μM) had significantly higher fresh weight, average root length, significantly reduced cell size, increased cell number, and increased cytoskeleton filaments in single cells. The expression pattern of one of 10 Tree bean Dynamic Balance Movement Related Protein (TbDMP), TbDMP was consistent with the sodium ion-stress alleviation by NAM. Using TbDMP as bait, Dynamic Balance Movement Related Kinase Protein (TbDBK) was determined to interact with TbDMP by screening the tree bean root cDNA library in yeast. Biochemical experiments showed that NAM enhanced the interaction between the two proteins which promoted resist sodium ion stress resistance. This study provides evidence of a pathway through which the skeleton participates in NAM signaling.
树豆（Cajanus cajan (L.) Millsp.）是一种具有经济价值的木本植物，主要种植在热带和亚热带地区，被认为是一种重要的食用豆类（或豆类）作物，面临着严重的钠离子胁迫。NAM（N-乙酰-5-甲氧基色胺）已被牵连到植物的非生物和生物胁迫耐受中。然而，NAM 在钠离子胁迫耐受中的作用尚未确定。在这项研究中，研究了 NAM 对经济价值高的木本植物物种的影响，该物种在 40mM 钠离子胁迫下处理 3 天。用 NAM 处理的植物（200μM）具有显著更高的鲜重、平均根长、显著减小的细胞大小、增加的细胞数量和单个细胞中增加的细胞骨架丝。10 个树豆动态平衡运动相关蛋白（TbDMP）之一的表达模式与 NAM 减轻钠离子胁迫的作用一致。使用 TbDMP 作为诱饵，通过筛选酵母中的树豆根 cDNA 文库，确定了动态平衡运动相关激酶蛋白（TbDBK）与 TbDMP 相互作用。生化实验表明，NAM 增强了这两种蛋白之间的相互作用，从而促进了抵抗钠离子胁迫的能力。这项研究提供了证据表明骨架参与了 NAM 信号通路。
