Ben Romdhane Walid, Ben-Saad Rania, Meynard Donaldo, Verdeil Jean-Luc, Azaza Jalel, Zouari Nabil, Fki Lotfi, Guiderdoni Emmanuel, Al-Doss Abdullah, Hassairi Afif
Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box 2460, 11451 Riyadh, Saudi Arabia.
Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax, University of Sfax, B.P 1177, 3018 Sfax, Tunisia.
Int J Mol Sci. 2017 Mar 24;18(4):692. doi: 10.3390/ijms18040692.
We report here the isolation and functional analysis of gene encoding a member of the PMP3 protein family. In , is highly induced by abscisic acid (ABA), cold, salt, and osmotic stresses. Transgenic tobacco expressing exhibited enhanced tolerance to salt, osmotic, H₂O₂, heat and freezing stresses at the seedling stage. Under greenhouse conditions, the transgenic plants showed a higher level of tolerance to drought than to salinity. Noteworthy, plants yielded two- and five-fold more seeds than non-transgenic plants (NT) under salt and drought stresses, respectively. The leaves of plants accumulated lower Na⁺ but higher K⁺ and Ca than those of NT plants. Tolerance to osmotic and salt stresses was associated with higher membrane stability, low electrolyte leakage, and improved water status. Finally, accumulation of in tobacco altered the regulation of some stress-related genes in either a positive (, , , and ) or negative ( and ) manner that could be related to the observed tolerance. These results suggest that confers stress tolerance in tobacco through maintenance of ion homeostasis, increased membrane integrity, and water status. The observed tolerance may be due to a direct or indirect effect of on the expression of stress-related genes which could stimulate an adaptive potential not present in NT plants.
我们在此报告编码PMP3蛋白家族成员的基因的分离及功能分析。在[具体情况未提及]中，[该基因]受脱落酸（ABA）、低温、盐和渗透胁迫高度诱导。在幼苗期，表达[该基因]的转基因烟草对盐、渗透、H₂O₂、热和冷冻胁迫表现出增强的耐受性。在温室条件下，转基因植株对干旱的耐受性高于对盐度的耐受性。值得注意的是，在盐胁迫和干旱胁迫下，[转基因]植株分别比非转基因植株（NT）多产两倍和五倍的种子。[转基因]植株叶片积累的Na⁺较低，但K⁺和Ca比NT植株高。对渗透和盐胁迫的耐受性与更高的膜稳定性、低电解质渗漏和改善的水分状况有关。最后，[该基因]在烟草中的积累以正向（[基因名称列举1]、[基因名称列举2]、[基因名称列举3]和[基因名称列举4]）或负向（[基因名称列举5]和[基因名称列举6]）方式改变了一些胁迫相关基因的调控，这可能与观察到的耐受性有关。这些结果表明，[该基因]通过维持离子稳态、增加膜完整性和水分状况赋予烟草胁迫耐受性。观察到的耐受性可能是由于[该基因]对胁迫相关基因表达的直接或间接影响，这可能刺激了NT植株中不存在的适应潜力。
