非粘性薄膜金属玻璃涂层注射器针的临床前研究。

Preclinical studies of non-stick thin film metallic glass-coated syringe needles.

机构信息

Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, No. 43, Keelung Rd., Sec.4, Da'an Dist., Taipei City, 10607, Taiwan.

Adjunct Appointment to the National Defense Medical Center, Taipei City, 11490, Taiwan.

出版信息

Sci Rep. 2020 Nov 20;10(1):20313. doi: 10.1038/s41598-020-77008-y.

DOI:10.1038/s41598-020-77008-y

PMID:33219243

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7679377/

Abstract

Our objective in this study was to determine the biocompatibility and hemocompatibility of thin film metallic glass (TFMG) and its potential use in hypodermic needles for intramuscular or intravenous injection. Mouse and rabbit models were employed under approval from the Institutional Animal Care and Use Committee (n = 5/group, two groups in total for both animal models). Platelet-rich plasma (PRP) was collected from the whole blood of rabbits (ear vein) without anti-coagulant for use in in vitro coagulation tests. Histological analysis and optical microscopy were used to assess the endothelial structure of the inner lining of veins after being punctured with needles and detained for 3 days. Histological analysis of ear vein sections revealed that the extent of endothelial damage after puncturing with a TFMG-coated needle was 33% less than that produced by bare needles. Our results confirm that the deposition of a thin TFMG layer (e.g., ZrCuAlTa) on the surface of hypodermic needle can have remarkably clinical benefits, including anti-adhesion, reduced invasion, and minimal endothelial damage. Our results also confirm the good biocompatibility and hemocompatibility of the TFMG coatings.

摘要

本研究旨在确定薄膜金属玻璃（TFMG）的生物相容性和血液相容性，及其在皮下注射针中用于肌肉内或静脉内注射的潜在用途。在机构动物护理和使用委员会的批准下（每组 n=5/组，两种动物模型共两组），使用小鼠和兔模型进行研究。从兔子（耳静脉）的全血中收集无抗凝剂的富含血小板血浆（PRP），用于体外凝血试验。组织学分析和光学显微镜用于评估静脉内膜在被针穿刺并留置 3 天后的内皮结构。耳静脉切片的组织学分析表明，涂覆有 TFMG 的针穿刺后的内皮损伤程度比裸针小 33%。我们的结果证实，在皮下注射针表面沉积一层薄薄的 TFMG 层（例如 ZrCuAlTa）可带来显著的临床益处，包括抗粘连、减少侵袭和最小化内皮损伤。我们的结果还证实了 TFMG 涂层具有良好的生物相容性和血液相容性。