Department of Biomedical Engineering, College of Engineering, Peking University, Beijing 100871, China.
College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.
ACS Appl Mater Interfaces. 2021 Feb 3;13(4):4835-4843. doi: 10.1021/acsami.0c19790. Epub 2021 Jan 21.
A wide range of biomedical devices are being used to treat cardiovascular diseases, and thus they routinely come into contact with blood. Insufficient hemocompatibility has been found to impair the functionality and safety of these devices through the activation of blood coagulation and the immune system. Numerous attempts have been made to develop surface modification approaches of the cardiovascular devices to improve their hemocompatibility. However, there are still no ideal "blood-friendly" coating materials, which possess the desired hemocompatibility, tissue compatibility, and mechanical properties. As a novel multifunctional material, graphene has been proposed for a wide range of biomedical applications. The chemical inertness, atomic smoothness, and high durability make graphene an ideal candidate as a surface coating material for implantable devices. Here, we evaluated the hemocompatibility of a graphene film prepared on quartz glasses (Gra-glasses) from a direct chemical vapor deposition process. We found that the graphene coating, which is free of transfer-mediating polymer contamination, significantly suppressed platelet adhesion and activation, prolonged coagulation time, and reduced thrombosis formation. We attribute the excellent antithrombogenic properties of the Gra-glasses to the low surface roughness, low surface energy (especially the low polar component of the surface energy), and the negative surface charge of the graphene film. Given these excellent hemocompatible properties, along with its chemical inertness, high durability, and molecular impermeability, a graphene film holds great promise as an antithrombogenic coating for next-generation cardiovascular devices.
大量的生物医学设备被用于治疗心血管疾病，因此它们经常与血液接触。研究发现，较差的血液相容性会通过激活血液凝固和免疫系统来损害这些设备的功能和安全性。人们已经尝试了许多方法来开发心血管设备的表面改性方法，以提高其血液相容性。然而，仍然没有理想的“亲血”涂层材料，这些材料具有所需的血液相容性、组织相容性和机械性能。作为一种新型多功能材料，石墨烯已被提出用于广泛的生物医学应用。化学惰性、原子平整度和高耐久性使石墨烯成为植入式设备表面涂层材料的理想选择。在这里，我们评估了从直接化学气相沉积过程制备的石英玻璃上的石墨烯膜（Gra-玻璃）的血液相容性。我们发现，无转移介导聚合物污染的石墨烯涂层可显著抑制血小板黏附和激活，延长凝血时间，减少血栓形成。我们将 Gra-玻璃的优异抗血栓形成性能归因于石墨烯膜的低表面粗糙度、低表面能（特别是表面能的低极性分量）和负表面电荷。鉴于这些优异的血液相容性特性，以及其化学惰性、高耐久性和分子不可渗透性，石墨烯膜有望成为下一代心血管设备的抗血栓形成涂层。
