加压液体萃取和超临界流体萃取从费约果叶提取物中获得的抗氧化和抗菌化合物。
Antioxidant and antibacterial compounds from feijoa leaf extracts obtained by pressurized liquid extraction and supercritical fluid extraction.
机构信息
Laboratory of Thermodynamics and Supercritical Technology (LATESC), Department of Chemical and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), CEP 88040-900 Florianópolis, SC, Brazil.
Laboratory of Thermodynamics and Supercritical Technology (LATESC), Department of Chemical and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), CEP 88040-900 Florianópolis, SC, Brazil.
出版信息
Food Chem. 2021 May 15;344:128620. doi: 10.1016/j.foodchem.2020.128620. Epub 2020 Nov 13.
This work investigated the efficiency of pressurized liquid extraction (PLE) and supercritical fluid extraction with cosolvent (SFE) in obtaining feijoa leaf extracts with high antioxidant and antibacterial activities. PLE was performed in customized equipment with environmentally friendly solvents, at 40/80 °C, in dynamic and static mode, and SFE was carried out for 210 min at 30 MPa, 55 °C and 15% ethanol-water as cosolvent. PLE extract (80 °C/ethanol-water/dynamic) provided the highest yield, total phenolic content, and antioxidant activities, but it was not effective as antibacterial agent. In contrast, SFE extract exhibited effectiveness against S. aureus, E. coli, and S. typhimurium, with minimum inhibitory concentration values from 14,211 to 3,553 µg.mL. Finally, gallic acid, catechin and isoquercetin were the major phenolics identified by liquid chromatography. Our findings revealed that feijoa leaf extracts by PLE and SFE have remarkable bioactivity, presenting a great potential to be used as natural food additives.
本研究旨在比较加压液体萃取（PLE）和带溶剂的超临界流体萃取（SFE）在获得具有高抗氧化和抗菌活性的费约果叶提取物方面的效率。PLE 在定制设备中进行，使用环保溶剂，在 40/80°C 的动态和静态模式下进行，SFE 在 30 MPa、55°C 和 15%乙醇-水作为共溶剂的条件下进行 210 分钟。PLE 提取物（80°C/乙醇-水/动态）提供了最高的产率、总酚含量和抗氧化活性，但作为抗菌剂效果不佳。相比之下，SFE 提取物对金黄色葡萄球菌、大肠杆菌和鼠伤寒沙门氏菌具有有效性，最小抑菌浓度值为 14,211 至 3,553µg.mL。最后，通过液相色谱鉴定了 PLE 和 SFE 提取物中的主要酚类物质为没食子酸、儿茶素和异槲皮苷。我们的研究结果表明，费约果叶的 PLE 和 SFE 提取物具有显著的生物活性，具有作为天然食品添加剂的巨大潜力。
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