Qutieshat Abubaker S, Al-Hiyasat Ahmad S, Islam Mohammad R
Conservative Dentistry Department, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
Unit of Cell and Molecular Biology, Dundee Dental School, University of Dundee, Dundee, Scotland, UK.
J Conserv Dent. 2020 Jan-Feb;23(1):15-20. doi: 10.4103/JCD.JCD_274_20. Epub 2020 Oct 10.
The potential of graphene-based materials to improve the physiomechanical properties of Portland cement-based materials without compromising biocompatibility is of interest to dental researchers and remains to be discovered.
This study investigated the effects of adding graphene oxide nanoplatelets (GONPs) on the surface microhardness and biocompatibility of Portland cement.
Three prototype Portland cement powder formulations were prepared by adding 0, 1, and 3 wt % GONPs in powder form to Portland cement. Prototype cement specimens were in the form of disks, with a diameter of 10 mm and a thickness of 2 mm. In experiment 1, surface microhardness was measured using the through indenter viewing hardness tester, 20 surface hardness values were obtained from all specimens. In experiment 2, Balb/C 3T3 fibroblasts were cultured with the material disks and the viability of cells was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.
The data were analyzed using the analysis of variance followed by Dunnett test (α = 0.05) or Tukey test (α = 0.05).
In response to material disks, the addition of 1 wt % GONPs had a proliferative effect on cells at day 3 and day 7 with a significant difference from the control. The addition of 3 wt % GONPs showed a remarkable increase in surface microhardness; however, it exhibited initial cytotoxicity.
The addition of 1 wt % GONPs to Portland cement improved surface microhardness without compromising biocompatibility; therefore, it has a greater potential for dental applications. The results of this work give other researchers leads in future assessments of this prototype material.
基于石墨烯的材料在不影响生物相容性的情况下改善波特兰水泥基材料物理力学性能的潜力，引起了牙科研究人员的兴趣，有待进一步探索。
本研究调查添加氧化石墨烯纳米片（GONPs）对波特兰水泥表面显微硬度和生物相容性的影响。
制备三种波特兰水泥粉末配方，分别向波特兰水泥中添加0、1和3 wt%的粉末状GONPs。原型水泥试件为圆盘状，直径10 mm，厚度2 mm。在实验1中，使用贯穿压头式硬度测试仪测量表面显微硬度，从所有试件中获取20个表面硬度值。在实验2中，将Balb/C 3T3成纤维细胞与材料圆盘一起培养，并使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法评估细胞活力。
数据采用方差分析，随后进行Dunnett检验（α = 0.05）或Tukey检验（α = 0.05）。
对于材料圆盘，添加1 wt%的GONPs在第3天和第7天对细胞有增殖作用，与对照组有显著差异。添加3 wt%的GONPs使表面显微硬度显著增加；然而，它表现出初始细胞毒性。
向波特兰水泥中添加1 wt%的GONPs可提高表面显微硬度，同时不影响生物相容性；因此，它在牙科应用中具有更大的潜力。本研究结果为其他研究人员对这种原型材料的未来评估提供了方向。
