Qutieshat Abubaker S, Al-Hiyasat Ahmad S, Darmani Homa
Department of Conservative Dentistry, Faculty of Dentistry, Jordan University of Science and Technology, Irbid, Jordan.
Department of Applied Biological Sciences, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan.
J Conserv Dent. 2019 May-Jun;22(3):249-254. doi: 10.4103/JCD.JCD_552_18.
The demand for novel Portland cement (PC)-based formulations to be used in dental applications is ever increasing in viewing the foregoing knowledge on the favorable effects of these formulations on cellular proliferation and healing, leading to treatment success.
This study investigated the effect of white and gray mineral trioxide aggregate (W-MTA and G-MTA) and white and gray Jordanian PC (W-PC and G-PC) in their raw state on the viability of Balb/C 3T3 fibroblasts using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
Materials were prepared in the form of disks, with a diameter of 5 mm and a thickness of 2 mm. In the first experiment, Balb/C 3T3 fibroblasts were cultured with the material disks using culture plate inserts. In the second experiment, material elutes were added to cultured cells. The elutes were prepared by adding 2 ml serum-free media to 10 disks of each material and then incubated at 37°C for different time intervals. Material elutes were analyzed using ion chromatography for traces of calcium. The data were analyzed using analysis of variance followed by Dunnett test (α = 0.05) or Tukey test (α = 0.05).
In response to material disks, G-PC had a proliferative effect on cells at day 1 and day 2 with a significant difference from the control at day 1. G-MTA reduced cell viability with a significant difference from the control level at day 2. Elutes of PC showed biocompatible and even proliferative effects on Balb/C 3T3 fibroblasts. Calcium ions were found to leach continuously over the measurement period for all the materials tested in this work.
Jordanian PC in its raw state was found to be biocompatible, and the results of this work give promise of its wider use as a base for further development to improve the physiochemical and mechanical properties of the material.
鉴于上述关于这些配方对细胞增殖和愈合的有利影响的知识，用于牙科应用的新型波特兰水泥（PC）基配方的需求不断增加，这会带来治疗成功。
本研究使用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）测定法，研究白色和灰色矿物三氧化物聚合体（W-MTA和G-MTA）以及白色和灰色约旦PC（W-PC和G-PC）在其原始状态下对Balb/C 3T3成纤维细胞活力的影响。
材料制备成直径5毫米、厚度2毫米的圆盘形式。在第一个实验中，使用培养板插入物将Balb/C 3T3成纤维细胞与材料圆盘一起培养。在第二个实验中，将材料洗脱液添加到培养的细胞中。洗脱液通过向每种材料的10个圆盘加入2毫升无血清培养基制备，然后在37°C下孵育不同时间间隔。使用离子色谱分析材料洗脱液中的钙痕量。数据使用方差分析，随后进行Dunnett检验（α = 0.05）或Tukey检验（α = 0.05）进行分析。
对于材料圆盘，G-PC在第1天和第2天对细胞有增殖作用，在第1天与对照组有显著差异。G-MTA在第2天降低了细胞活力，与对照水平有显著差异。PC的洗脱液对Balb/C 3T3成纤维细胞显示出生物相容性甚至增殖作用。在本工作中测试的所有材料的测量期间，发现钙离子持续渗出。
发现原始状态的约旦PC具有生物相容性，这项工作的结果有望使其更广泛地用作进一步开发的基础，以改善材料 的物理化学和机械性能。
