Kakani Abhijeet K, Veeramachaneni Chandrasekhar
Departments of Conservative Dentistry and Endodontics, Nanded Rural Dental College and Research Center, Nanded, Maharashtra, India.
Malla Reddy Dental College for Women, Hyderabad, Telangana, India.
J Conserv Dent. 2020 Jan-Feb;23(1):62-65. doi: 10.4103/JCD.JCD_371_19. Epub 2020 Oct 10.
The present study aimed to evaluate and compare the sealing ability of mineral trioxide aggregate (MTA)-Angelus, Biodentine™, and EndoSequence cement in furcation perforations using protein leakage assessment.
The present study was conducted using seventy extracted human maxillary and mandibular molars with intact furcation. The samples were randomly allocated into three groups ( = 20) based on repair material used: Group 1 - MTA-Angelus, Group 2 - Biodentine™, and Group 3 - EndoSequence. Two additional groups served as positive and negative controls ( = 5). Using the leakage assessment apparatus, the presence of protein was detected with a reagent (Coomassive Brilliant Blue) every day for 60 days. One-way ANOVA and Tukey's test were used for statistical analysis using SPSS software.
All the groups exhibited protein leakage from day 1. Biodentine showed minimum (0.1201 mg/ml), MTA showed maximum (0.3738 mg/ml), and EndoSequence had intermediate (0.2465 mg/ml) leakage. None in the negative control and all of the positive control specimens leaked during the experimental period of 60 days.
The newer biomaterials, Biodentine and Endosequence with better handling properties, could be used as alternatives to MTA-Angelus while repairing furcation perforations.
本研究旨在通过蛋白质渗漏评估来评价和比较三氧化矿物凝聚体（MTA）-安捷鲁斯、生物陶瓷（Biodentine™）和EndoSequence根管充填剂在根分叉穿孔处的封闭能力。
本研究使用70颗完整根分叉的拔除人上颌和下颌磨牙进行。根据使用的修复材料将样本随机分为三组（每组n = 20）：第1组 - MTA-安捷鲁斯，第2组 - 生物陶瓷（Biodentine™），第3组 - EndoSequence。另外两组作为阳性和阴性对照（每组n = 5）。使用渗漏评估装置，每天用试剂（考马斯亮蓝）检测蛋白质的存在，持续60天。使用SPSS软件进行单因素方差分析和Tukey检验进行统计分析。
所有组从第1天起均出现蛋白质渗漏。生物陶瓷的渗漏量最小（0.1201mg/ml），MTA的渗漏量最大（0.3738mg/ml），EndoSequence的渗漏量居中（0.2465mg/ml）。在60天的实验期内，阴性对照组无一渗漏，阳性对照组全部渗漏。
生物陶瓷和EndoSequence等具有更好操作性能的新型生物材料在修复根分叉穿孔时可作为MTA-安捷鲁斯的替代品。
