Effect of on Biofilm Formation and Antibiotic Susceptibility in .

infections are notoriously difficult to be treated and newer treatment options are required. () and its main compound berberine are frequently used to treat bacterial and viral infections. In this study, the susceptibility of to extract and berberine was assessed by minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) evaluation. The effects of and berberine on biofilm formation and antibiotic susceptibility in were observed. at concentrations of MIC (1.5 mg/mL) and 2 × MIC (3.0 mg/mL) and berberine at ½ × MIC (0.125 mg/mL) demonstrated a strong inhibition of biofilm formation. Concentration of at ½ × MIC resulted in a significant reduction in MICs of trimethoprim/sulfamethoxazole (TMP/SXT), clarithromycin (CLA), and linezolid (LZD). Similarly, ½ × MIC berberine had a significant effect on the MIC reductions of nine antibiotics including TMP/SXT, CLA, and LZD. Notably, the resistance level MIC of LZD against was reversed to a susceptible level by treatment with either or berberine. Therefore, and berberine have the potential to produce a synergistic antimycobacterial effect, reduce biofilm formation, and decrease antibacterial resistance to LZD in .