异常表达的长链非编码RNA可改变宿主对SARS-CoV-2感染的反应及病毒增殖。
Perversely expressed long noncoding RNAs can alter host response and viral proliferation in SARS-CoV-2 infection.
作者信息
Turjya Rafeed Rahman, Khan Md Abdullah-Al-Kamran, Mir Md Khademul Islam Abul Bashar
机构信息
Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.
Department of Mathematics & Natural Sciences, BRAC University, Dhaka, Bangladesh.
出版信息
Future Virol. 2020 Sep;15(9):577-593. doi: 10.2217/fvl-2020-0188.
BACKGROUND
Regulatory roles of long noncoding RNAs (lncRNAs) during viral infection has become more evident in last decade, but are yet to be explored for SARS-CoV-2.
MATERIALS & METHODS: We analyzed RNA-seq dataset of SARS-CoV-2 infected lung epithelial cells to identify differentially expressed genes.
RESULTS
Our analyses uncover 21 differentially expressed lncRNAs broadly involved in cell survival and regulation of gene expression. These lncRNAs can directly interact with six differentially expressed protein-coding genes, and ten host genes that interact with SARS-CoV-2 proteins. Also, they can block the suppressive effect of nine microRNAs induced in viral infections.
CONCLUSION
Our investigation determines that deregulated lncRNAs in SARS-CoV-2 infection are involved in viral proliferation, cellular survival, and immune response, ultimately determining disease outcome.
背景
在过去十年中，长链非编码RNA（lncRNA）在病毒感染过程中的调控作用愈发明显，但针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的相关作用仍有待探索。
材料与方法
我们分析了SARS-CoV-2感染的肺上皮细胞的RNA测序数据集，以鉴定差异表达基因。
结果
我们的分析发现了21种差异表达的lncRNA，它们广泛参与细胞存活和基因表达调控。这些lncRNA可直接与6个差异表达的蛋白质编码基因以及10个与SARS-CoV-2蛋白相互作用的宿主基因相互作用。此外，它们还可以阻断病毒感染诱导的9种微小RNA的抑制作用。
结论
我们的研究确定，SARS-CoV-2感染中失调的lncRNA参与病毒增殖、细胞存活和免疫反应，最终决定疾病结局。
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