利用计算方法从广食性鳞翅目害虫斜纹夜蛾中鉴定效应蛋白。
In silico identification of effector proteins from generalist herbivore Spodoptera litura.
机构信息
National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, 110067, India.
Present Address-Population Ecology Group, Institute of Ecology and Evolution, Friedrich Schiller University Jena, Dornburger Straße 159, 07743, Jena, Germany.
出版信息
BMC Genomics. 2020 Nov 23;21(1):819. doi: 10.1186/s12864-020-07196-4.
BACKGROUND
The common cutworm, Spodoptera litura Fabricius is a leaf and fruit feeding generalist insect of the order Lepidoptera and a destructive agriculture pest. The broad host range of the herbivore is due to its ability to downregulate plant defense across different plants. The identity of Spodoptera litura released effectors that downregulate plant defense are largely unknown. The current study aims to identify genes encoding effector proteins from salivary glands of S. litura (Fab.).
RESULTS
Head and salivary glands of Spodoptera litura were used for de-novo transcriptome analysis and effector prediction. Eight hundred ninety-nine proteins from the head and 330 from salivary gland were identified as secretory proteins. Eight hundred eight proteins from the head and 267 from salivary gland proteins were predicted to be potential effector proteins.
CONCLUSIONS
This study is the first report on identification of potential effectors from Spodoptera litura salivary glands.
背景
斜纹夜蛾（Spodoptera litura Fabricius）是鳞翅目夜蛾科的一种多食性昆虫，以叶片和果实为食，是一种具有破坏性的农业害虫。这种食草动物的广泛宿主范围是由于其能够在不同植物中下调植物防御。斜纹夜蛾释放的下调植物防御的效应物的身份在很大程度上是未知的。本研究旨在从斜纹夜蛾（Fab.）的唾液腺中鉴定编码效应蛋白的基因。
结果
使用斜纹夜蛾的头部和唾液腺进行从头转录组分析和效应子预测。从头部鉴定出 899 种分泌蛋白和 330 种唾液腺分泌蛋白。从头部鉴定出 808 种潜在效应蛋白和 267 种唾液腺蛋白。
结论
本研究首次报道了从斜纹夜蛾唾液腺中鉴定潜在效应物。
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