Echeverry-Solarte Morgan, Kumar Ajay, Kianian Shahryar, Mantovani Eder E, McClean Phillip E, Deckard Edward L, Elias Elias, Simsek Senay, Alamri Mohammed S, Hegstad Justin, Schatz Blaine, Mergoum Mohamed
Dep. of Plant Sciences, North Dakota State Univ., P.O. Box 6050, Fargo, ND, 58108-6050.
USDA-ARS - Cereal Disease Laboratory, 1551 Lindig St., Univ. of Minnesota, St. Paul, Minnesota, 55108.
Plant Genome. 2015 Jul;8(2):eplantgenome2014.12.0089. doi: 10.3835/plantgenome2014.12.0089.
In wheat, exotic genotypes harbor a broad range of spike-related traits, and can be used as a source of new genes for germplasm enhancement in wheat breeding programs. In the present study, a population of 163 recombinant inbred lines was derived from a cross between an elite line (WCB414) and an exotic line (WCB617) with branched spike (supernumerary spikelet; SS) head morphology. The population was evaluated over four to six environments to identify quantitative trait loci (QTL) associated with nine spike-related traits and 10 agronomic traits. A genetic map consisting of 939 diversity arrays technology (DArT) markers was constructed. Composite interval mapping identified a total of 143 QTL located on 17 different wheat chromosomes and included 33 consistent and definitive QTL. The amount of phenotype variation explained (PVE) by individual QTL ranged from 0.61 to 91.8%. One major QTL for glume pubescence was located in a QTL-rich region on the short arm of chromosome 1A, where loci for other traits such as for kernels per spike (KS) and spike length (SL) were also identified. Similarly, a cluster of QTL associated with yield-related, agronomic and spike-related traits contributing up to 40.3% of PVE was found on the short arm of chromosome 2D, in the vicinity of a major QTL for SS-related traits. Consistent and major QTL identified in the present study may be useful in marker-assisted breeding programs to facilitate transfer of desirable alleles into other germplasm. Desirable QTL alleles were also contributed by the exotic line, suggesting the possibility of enriching the breeding germplasm with alleles from SS genotypes.
在小麦中，外来基因型具有广泛的穗部相关性状，可作为小麦育种计划中种质改良新基因的来源。在本研究中，一个由163个重组自交系组成的群体源自一个优良品系（WCB414）与一个具有分枝穗（多余小穗；SS）穗形态的外来品系（WCB617）之间的杂交。该群体在四到六个环境中进行评估，以鉴定与九个穗部相关性状和十个农艺性状相关的数量性状位点（QTL）。构建了一个由939个多样性阵列技术（DArT）标记组成的遗传图谱。复合区间作图共鉴定出位于17条不同小麦染色体上的143个QTL，其中包括33个一致且明确的QTL。单个QTL解释的表型变异量（PVE）范围为0.61%至91.8%。一个控制颖壳茸毛的主要QTL位于1A染色体短臂上的一个QTL富集区域，在该区域还鉴定出了其他性状的位点，如每穗粒数（KS）和穗长（SL）。同样，在2D染色体短臂上，靠近一个与SS相关性状的主要QTL附近，发现了一组与产量相关、农艺和穗部相关性状相关的QTL，其对PVE的贡献率高达40.3%。本研究中鉴定出的一致且主要的QTL可能有助于标记辅助育种计划，促进将理想等位基因转移到其他种质中。外来品系也贡献了理想的QTL等位基因，这表明有可能用来自SS基因型的等位基因丰富育种种质。
