Department of Gynecology, The First Hospital of China Medical University, 155 Nanjing Street, Shenyang City, Liaoning Province 110001, China.
Discipline Inspection Commission, China Medical University, No. 77 Puhe Road, Shenyang North New Area, Shenyang, Liaoning Province 110122. China.
Bioorg Chem. 2021 Jan;106:104468. doi: 10.1016/j.bioorg.2020.104468. Epub 2020 Nov 10.
At the moment, metallic nanoparticles especially copper nanoparticles are administrated for the cure of different disorders, such as tumor and cancer. In recent years, many chemotherapeutic supplements have been formulated by copper nanoparticles. In the present study, copper nanoparticles were prepared and synthesized in aqueous medium using Camellia sinensis leaf extract. The as-prepared CuO nanoparticles was thoroughly characterized using XRD, FT-IR, FESEM, EDX, TEM and X-ray elemental mapping techniques. The as-synthesized CuO/C. sinensis NPs applied as novel nanocatalyst for the synthesis of annulated fused pyrano[2,3-d]pyrimidinones via a one-pot, three-component condensation of a barbituric acid, aromatic aldehydes, and malonitrile or ethylcyanoacetate under mild condition at 25 °C. Main properties of this facile method are the involves an easy work-up procedure, avoidance of hazardous or polluting chemicals, significant yields under mild conditions, and one-pot reaction. We assessed the anti-human ovarian cancer potentials of these nanoparticles against Caov-3, SW-626, and SK-OV-3 cell lines. For investigating the antioxidant activities of CuCl⋅2HO, C. sinensis, and copper nanoparticles, the DPPH free radical test was used. For the determining of anti-human ovarian cancer properties of CuCl⋅2HO, Camellia sinensis leaf aqueous extract, copper nanoparticles, and Carboplatin (Standard positive control), MTT assay was used on normal (HUVECs) and human ovarian cancer (Caov-3, SW-626, and SK-OV-3) cell lines. Copper nanoparticles had high cell death and anti-human ovarian cancer properties against Caov-3, SW-626, and SK-OV-3 cell lines. Among the above cell lines, the best result was gained in the cell line of SW-626. According to the above findings, it looks copper nanoparticles green-synthesized by Camellia sinensis leaf aqueous extract have the potential to be used as a chemotherapeutic material for human ovarian cancers.
目前，金属纳米粒子特别是铜纳米粒子被用于治疗各种疾病，如肿瘤和癌症。近年来，许多化疗药物补充剂都是由铜纳米粒子制成的。在本研究中，使用茶树叶片提取物在水介质中制备和合成了铜纳米粒子。使用 XRD、FT-IR、FESEM、EDX、TEM 和 X 射线元素映射技术对所制备的 CuO 纳米粒子进行了彻底的表征。所合成的 CuO/茶树 NPs 被用作新型纳米催化剂，通过一锅法、三组分缩合，在 25°C 温和条件下，从巴比妥酸、芳香醛、丙二腈或氰乙酸乙酯合成了稠合的并环[2,3-d]嘧啶酮。这种简便方法的主要特点是操作简单、避免使用危险或污染性化学物质、在温和条件下产率高、以及一锅反应。我们评估了这些纳米粒子对 Caov-3、SW-626 和 SK-OV-3 细胞系的抗人卵巢癌潜力。为了研究 CuCl⋅2HO、茶树和铜纳米粒子的抗氧化活性，使用 DPPH 自由基测试。为了确定 CuCl⋅2HO、茶树叶片水提物、铜纳米粒子和卡铂（标准阳性对照）对人卵巢癌的抗药性，MTT 法用于正常（HUVECs）和人卵巢癌细胞（Caov-3、SW-626 和 SK-OV-3）。铜纳米粒子对 Caov-3、SW-626 和 SK-OV-3 细胞系具有高细胞死亡和抗人卵巢癌特性。在上述细胞系中，在 SW-626 细胞系中获得了最佳结果。根据上述发现，看起来由茶树叶片水提物绿色合成的铜纳米粒子有潜力用作治疗人类卵巢癌的化疗药物。
