Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, Tamil Nadu, India.
Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632014, Tamil Nadu, India.
J Photochem Photobiol B. 2017 Jun;171:133-138. doi: 10.1016/j.jphotobiol.2017.05.001. Epub 2017 May 2.
The eco-friendly synthesis of nanoparticles through green route from plant extracts have renowned a wide range of application in the field of modern science, due to increased drug efficacy and less toxicity in the nanosized mediated drug delivery model. In the present study, our research groups have biosynthesized the stable and cost effective copper oxide nanoparticles (CuO NPs) from the leaves of (Ormocarpum cochinchinense) O. cochinchinense. The synthesis of crystalline CuO NPs from the leaf extract of O. cochinchinense were confirmed by various analytical techniques like UV-Visible Spectroscopy (UV-Vis), Fourier-Transform Infrared Spectroscopy (FT-IR), X-Ray Diffractometer (XRD), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Selected Area Electron Diffraction (SAED) pattern. Further the synthesized CuO NPs were screened for anticancer activity on human colon cancer cell lines (HCT-116) by MTT (3-(4,5-dimethyl-2-tiazolyl)-2,5-diphenyl-2-tetrazolium bromide) assay. The obtained result inferred that the synthesized CuO NPs demonstrated high anticancer cytotoxicity on human colon cancer cell lines (HCT-116) with IC value of 40μgmL were discussed briefly in this manuscript.
通过植物提取物进行绿色途径合成纳米粒子，由于在纳米介导的药物输送模型中提高了药物功效和降低了毒性，因此在现代科学领域得到了广泛的应用。在本研究中，我们的研究小组已经从（Ormocarpum cochinchinense）的叶子中生物合成了稳定且具有成本效益的氧化铜纳米粒子（CuO NPs）。O. cochinchinense。通过各种分析技术，如紫外-可见光谱（UV-Vis）、傅里叶变换红外光谱（FT-IR）、X 射线衍射仪（XRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和选区电子衍射（SAED）图谱，证实了从 O. cochinchinense 叶提取物中合成的结晶 CuO NPs。进一步，通过 MTT（3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2-四唑溴化物）测定法筛选了合成的 CuO NPs 对人结肠癌细胞系（HCT-116）的抗癌活性。获得的结果推断，合成的 CuO NPs 对人结肠癌细胞系（HCT-116）表现出高抗癌细胞毒性，IC 值为 40μgmL，在本文中进行了简要讨论。
