Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, 119071 Moscow, Russia.
Sensors (Basel). 2022 Dec 12;22(24):9724. doi: 10.3390/s22249724.
Assessment of the composition of meat-containing products is the task in demand due to their frequent deviations from declared recipes. The paper presents the developed test system for immunochromatographic determination of total meat content. The assay is based on the simultaneous use of monoclonal antibodies, which specifically interacts with mammalian skeletal troponin I, and polyclonal antibodies, which specifically detect bird immunoglobulin Y. To integrate the detection of both types of meat by the same test strip, the antibodies are mixed in the analytical zone of the test strip and in complex with a gold nanoparticle label. The chosen ratios of the antibodies for both mixtures provide the same contribution of different types of mammalian and bird raw materials of muscle tissues to the label binding. The test system demonstrates suitability for products containing beef, pork, rabbit, lamb, chicken, and turkey meat. The minimal detectable content of meat in samples is 0.1%. The samples for the testing are diluted 100 times, thus eliminating matrix effects, and providing high reproducibility of the color intensity for extracts of different compositions. The obtained results allow the recommendation of the developed test system for rapid on-site control of meat products.
评估含肉产品的组成是一项需求较高的任务，因为这些产品经常偏离所宣称的配方。本文介绍了一种用于免疫层析法定量测定总肉含量的测试系统。该测定法基于同时使用单克隆抗体和多克隆抗体，单克隆抗体特异性地与哺乳动物骨骼肌肌钙蛋白 I 相互作用，多克隆抗体特异性地检测鸟类免疫球蛋白 Y。为了通过同一条测试带同时检测两种类型的肉，将抗体混合在测试带的分析区，并与金纳米颗粒标记物结合。两种混合物中抗体的选择比例为不同类型的哺乳动物和鸟类肌肉组织的原始材料对标记物结合的贡献相同。该测试系统适用于含有牛肉、猪肉、兔肉、羊肉、鸡肉和火鸡肉的产品。样品中肉的最小可检测含量为 0.1%。用于测试的样品稀释 100 倍，从而消除了基质效应，并为不同组成的提取物提供了高重现性的颜色强度。所得结果允许推荐开发的测试系统用于快速现场控制肉类产品。
