Norton A J, Isaacson P G
Department of Histopathology, University College, London.
J Clin Pathol. 1987 Dec;40(12):1405-12. doi: 10.1136/jcp.40.12.1405.
Formalin fixed and paraffin wax embedded tissue from 85 well characterised cases of non-Hodgkin's lymphoma and Hodgkin's disease were studied using the avidin-biotin-peroxidase complex technique. Among the non-Hodgkin's lymphomas all cases of B cell lymphoma were reactive with L26, a monoclonal antibody which is as yet an unclustered pan B cell reagent, with the exception of pre-B cell acute lymphoblastic leukaemia and malignant lymphoma plasmacytic. Eighteen well characterised cases of T cell lymphoma, selected to include tumours previously shown to exhibit cross reactivity with antibodies to fixation resistant B cell related antigens, were similarly studied. Neoplastic cells in all but one case were unstained by L26. Twenty seven cases of Hodgkin's disease were also examined. In five cases all Reed-Sternberg cells and their variants were strongly stained by L26; only a proportion of Reed-Sternberg cells and their variants were recognised in a further five cases. Monoclonal antibody L26 promises to be a valuable reagent for the diagnosis of malignant lymphoma in routinely fixed and paraffin wax embedded tissues. Its advantage lies in its sensitivity and greater B cell specificity than any of the B cell related reagents currently available for the study of malignant lymphoma in fixed tissues.
采用抗生物素蛋白-生物素-过氧化物酶复合物技术，对85例特征明确的非霍奇金淋巴瘤和霍奇金病的福尔马林固定、石蜡包埋组织进行了研究。在非霍奇金淋巴瘤中，所有B细胞淋巴瘤病例均与L26呈反应性，L26是一种单克隆抗体，目前属于未分类的泛B细胞试剂，但前B细胞急性淋巴细胞白血病和浆细胞性恶性淋巴瘤除外。同样对18例特征明确的T细胞淋巴瘤病例进行了研究，这些病例被选入，包括先前显示与抗固定抗性B细胞相关抗原抗体呈交叉反应的肿瘤。除1例病例外，所有病例中的肿瘤细胞均未被L26染色。还检查了27例霍奇金病病例。在5例病例中，所有里德-施特恩贝格细胞及其变体均被L26强烈染色；在另外5例病例中，仅识别出一部分里德-施特恩贝格细胞及其变体。单克隆抗体L26有望成为诊断常规固定和石蜡包埋组织中恶性淋巴瘤的一种有价值的试剂。其优势在于其敏感性以及比目前用于研究固定组织中恶性淋巴瘤的任何B细胞相关试剂具有更高的B细胞特异性。
