Li Jinbing, Li Yuhang, Zhan Xiao-Yong, Ran Xinru, Tse Hung-Fat, Dang Shiying, Nie Yichu, Huang Ke
University of Chinese Academy of Sciences, Beijing 100049, China; CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Stem Cell Res. 2020 Dec;49:102090. doi: 10.1016/j.scr.2020.102090. Epub 2020 Nov 17.
GADD45A is a DNA damage and stressful growth arrest inducible protein, also it is shown to a be tumor suppressor gene and a chromatin relaxer associated with opening chromatin during the somatic reprogramming. However, its role in human embryonic stem cells and human embryonic stem cell modeled development has been merely documented. To illustrate the function of GADD45A in the human embryonic stem cell biology, we reported a GADD45A knockout human embryonic stem cell line by CRISPR/Cas9 mediated gene targeting. This cell line displayed normal karyotype, pluripotent stem cell marker expression and differentiation potential both in vivo and vitro.
GADD45A是一种DNA损伤和应激诱导生长停滞蛋白，它也被证明是一种肿瘤抑制基因，并且是一种在体细胞重编程过程中与开放染色质相关的染色质松弛剂。然而，其在人类胚胎干细胞和人类胚胎干细胞模型发育中的作用仅有文献记载。为了阐明GADD45A在人类胚胎干细胞生物学中的功能，我们报道了一种通过CRISPR/Cas9介导的基因靶向构建的GADD45A基因敲除人类胚胎干细胞系。该细胞系在体内和体外均表现出正常的核型、多能干细胞标志物表达和分化潜能。
