CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China; University of Chinese Academy of Sciences, Beijing 100049, China.
CAS Key Laboratory of Regenerative Biology, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
Stem Cell Res. 2021 May;53:102288. doi: 10.1016/j.scr.2021.102288. Epub 2021 Mar 13.
PARP1 encodes a chromatin-associated enzyme which responsible for post-translational poly(ADP-ribosyl)ation modification (Hsieh et al., 2017). It plays an important role in nucleotide excision repair, non-homologous end joining, DNA mismatch repair and many other DNA repair process. Also, PARP1 participates in inflammation and aging. However, its role in human embryonic stem cell biology has not been fully resolved. To clarify the function of PARP1 in human embryonic stem cells, we reported a PARP1 knockout human embryonic stem cell line, generated by CRISPR/Cas9 mediated gene targeting. This cell line shows normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro.
PARP1 编码一种与染色质相关的酶，负责翻译后多聚（ADP-核糖基）化修饰（Hsieh 等人，2017 年）。它在核苷酸切除修复、非同源末端连接、DNA 错配修复和许多其他 DNA 修复过程中发挥重要作用。此外，PARP1 还参与炎症和衰老。然而，其在人胚胎干细胞生物学中的作用尚未完全阐明。为了阐明 PARP1 在人胚胎干细胞中的功能，我们通过 CRISPR/Cas9 介导的基因靶向报告了 PARP1 敲除的人胚胎干细胞系。该细胞系显示正常核型、多能干细胞标志物表达和体外分化潜能。
