Department of General Practice, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.
Department of Hematology, The Seventh Affiliated Hospital, Sun Yat-Sen University, Shenzhen, China.
Stem Cell Res. 2022 Oct;64:102935. doi: 10.1016/j.scr.2022.102935. Epub 2022 Oct 5.
CD16A is a receptor for the Fc portion of immunoglobulin G, and is involved in the antibody dependent cellular cytotoxicity (ADCC) of nature killer cells(Zhu et al., 2020) and antibody dependent enhancement (ADE) of virus infections(Wan et al., 2020). However, the role of CD16A in human embryonic stem cell modeled development has been merely documented. Hence, to illustrate the role of CD16A in the human cell development, we reported a CD16A knockout human embryonic stem(hESC) cell line via CRISPR/Cas9 mediated gene knockout. The CD16A mutated cell line displayed normal karyotype, pluripotent stem cell marker gene expression and differentiation potential.
CD16A 是免疫球蛋白 G 的 Fc 部分的受体，参与自然杀伤细胞的抗体依赖细胞毒性 (ADCC)（Zhu 等人，2020 年）和病毒感染的抗体依赖增强 (ADE)（Wan 等人，2020 年）。然而，CD16A 在人类胚胎干细胞模型发育中的作用仅被记录下来。因此，为了阐明 CD16A 在人类细胞发育中的作用，我们通过 CRISPR/Cas9 介导的基因敲除报道了 CD16A 敲除的人类胚胎干细胞 (hESC) 细胞系。CD16A 突变的细胞系显示正常的核型、多能干细胞标记基因表达和分化潜能。
