Peng An, Lin Lianzhu, Zhao Mouming
School of Food Science and Engineering, South China University of Technology Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510641, China.
School of Food Science and Engineering, South China University of Technology Guangzhou 510641, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510641, China.
Food Res Int. 2020 Nov;137:109448. doi: 10.1016/j.foodres.2020.109448. Epub 2020 Jun 17.
This study aimed at obtaining precise quality control standards to efficiently screen Chrysanthemum morifolium Ramat. 'Boju' with strong xanthine oxidase (XO) inhibitory activity for developing C. morifolium 'Boju' extract based-hypouricemic functional food. The results indicated significant differences in volatile and phenolic profiles and XO inhibitory (XOI) activities of 31 batches of C. morifolium 'Boju' collected in herb/tea market. The minority of C. morifolium 'Boju' having abundant characteristic monoterpenoids and flavonoid aglycones showed strong XOI activities, while the majority of C. morifolium 'Boju' possessing few corresponding monoterpenoids and flavonoid aglycones exhibited moderate XOI activities. Seven XOI activity-directly related phenolics and ten XOI activity-indirectly related volatiles were identified by UPLC-ESI-QTOF/MS-based and HS-SPME-GC/MS-based spectrum-effect relationships, respectively. Three flavonoids (luteolin, apigenin and acacetin) identified as key XO inhibitors and four monoterpenoids (filifolone, α-terpineol, thymol and piperitenone) closely related to key XO inhibitors were screened for XOI activity-oriented quality control of C. morifolium 'Boju'.
本研究旨在获得精确的质量控制标准，以高效筛选具有强黄嘌呤氧化酶（XO）抑制活性的杭白菊‘亳菊’，用于开发基于杭白菊‘亳菊’提取物的降尿酸功能食品。结果表明，在草药/茶叶市场收集的31批次杭白菊‘亳菊’的挥发性成分、酚类成分和XO抑制（XOI）活性存在显著差异。少数含有丰富特征性单萜和黄酮苷元的杭白菊‘亳菊’表现出较强的XOI活性，而大多数含有少量相应单萜和黄酮苷元的杭白菊‘亳菊’表现出中等的XOI活性。分别通过基于超高效液相色谱-电喷雾-四极杆飞行时间质谱（UPLC-ESI-QTOF/MS）和顶空固相微萃取-气相色谱/质谱联用（HS-SPME-GC/MS）的谱效关系，鉴定出7种与XOI活性直接相关的酚类成分和10种与XOI活性间接相关的挥发性成分。筛选出3种被确定为关键XO抑制剂的黄酮类化合物（木犀草素、芹菜素和刺槐素）和4种与关键XO抑制剂密切相关的单萜类化合物（绵毛叶艾里莫芬酮、α-松油醇、百里香酚和胡椒烯酮），用于杭白菊‘亳菊’的XOI活性导向质量控制。
