Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur, 176062, India.
Department of Agricultural Biotechnology, CSK Himachal Pradesh Agricultural University, Palampur, 176062, India.
Food Res Int. 2020 Nov;137:109600. doi: 10.1016/j.foodres.2020.109600. Epub 2020 Jul 30.
Macrotyloma uniflorum (Lam.) Verdc. (family Fabaceae) is traditionally important pulse used for kidney stone degradation, cold,cough, asthma and in bronchial issues. It is well reported for the antilithiatic activity and nutritional profiling but no attempt has been made to find out the mechanism and nutrient responsible for the activity in its seed decoction. Therefore, lead high altitude variety responsible for the highest nutrient content and antilithiatic activity was selected for the study. Boiled spray dried extract (M), lead fractionation (Spray dried residual water fraction: M), and crude powder extract (M) were nutritionally profiled using proximate composition analysis, Atomic absorption spectroscopy (minerals and trace elements), Ion exchange chromatography (carbohydrate analysis) and NMR spectroscopy (polar and non polar metabolite analysis). These extracts were also screened for the antilithiatic activity and found significantly important. M showed more activity than M. Further, boiled extract was fractionated with different polarity of solvents (hexane, chloroform, n-butanol, residual water extract). All fractions and reference standard (cystone) were screened for the antilithiatic activity. M showed highest activity followed by ethyl acteate while hexane and chloroform did not show any activity in comparison to standards. Thus, lead fraction and market reference standard (cystone) were validated for the presence of nutritional components. Moreover, the selected nutritional components were individually and in combinations screened for the activity which revealed that kidney stone degradation was due to the synergetic action of components than the individual. The results clearly pave a way that Macrotyloma uniflorum is best for combating with malnutrition and kidney stone related problems.
锦鸡儿（Lam.）Verdc.（豆科）是一种传统的重要豆类，用于肾结石降解、感冒、咳嗽、哮喘和支气管问题。它具有良好的抗结石活性和营养分析，但尚未尝试找出其种子煎剂中活性的机制和营养成分。因此，选择了负责最高营养含量和抗结石活性的高原品种进行研究。使用近似成分分析、原子吸收光谱法（矿物质和微量元素）、离子交换色谱法（碳水化合物分析）和 NMR 光谱法（极性和非极性代谢物分析）对煮沸喷雾干燥提取物（M）、铅分级（喷雾干燥残余水级分：M）和粗粉提取物（M）进行了营养分析。这些提取物也被筛选出具有抗结石活性，并发现具有重要意义。M 比 M 表现出更高的活性。此外，用不同极性的溶剂（己烷、氯仿、正丁醇、残余水提取物）对煮沸提取物进行分级。所有级分和参比标准（结石通）均被筛选出具有抗结石活性。M 表现出最高的活性，其次是乙酸乙酯，而与标准品相比，己烷和氯仿没有任何活性。因此，对铅分级和市场参比标准（结石通）进行了营养成分存在的验证。此外，还单独和组合筛选了选定的营养成分的活性，结果表明肾结石降解是由于成分的协同作用而不是单个成分的作用。研究结果清楚地表明，锦鸡儿是对抗营养不良和肾结石相关问题的最佳选择。
