Key Laboratory of Shenzhen Respiratory Disease, Shenzhen Institute of Respiratory Disease, Shenzhen People's Hospital (The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College of Jinan University), Shenzhen, Guangdong, People's Republic of China.
Drug Des Devel Ther. 2020 Nov 17;14:5019-5027. doi: 10.2147/DDDT.S277126. eCollection 2020.
The present study was conducted to elucidate the protective effect of Casticin against chronic obstructive pulmonary disease (COPD) in rats.
The COPD in rats was induced by the controlled cigarette smoke, and CST (10, 20, and 30 mg/kg) was injected into the cigarette-smoke exposed rats. Blood was taken from the abdominal vein and centrifuged (1500×g, 4°C, 15min); plasma was collected and used for the determination of various biochemical parameters.
The results of the study suggested that CST significantly improved the lung functions of the rats in a dose-dependent manner. It also causes a reduction of white blood cells, neutrophils, and macrophages in BALF of rats. The plasma level of leptin and C-reactive protein together with pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) were also significantly restored to near to normal in CST-treated group. In Western blot analysis, CST causes significant inhibition of the NF-ĸB and iNOS pathway.
Our study demonstrated that the CST protects lungs against COPD via improving lung functions and inhibition of oxidative stress and inflammation.
本研究旨在阐明丁香脂素（Casticin）对大鼠慢性阻塞性肺疾病（COPD）的保护作用。
采用香烟烟雾暴露法建立大鼠 COPD 模型，用丁香脂素（10、20、30mg/kg）对香烟烟雾暴露的大鼠进行腹腔注射。从腹静脉采血，离心（1500×g，4°C，15min）；收集血浆，用于测定各种生化参数。
研究结果表明，丁香脂素能显著改善大鼠的肺功能，且呈剂量依赖性。它还能减少 BALF 中的白细胞、中性粒细胞和巨噬细胞。血浆瘦素和 C 反应蛋白水平以及促炎细胞因子（TNF-α、IL-1β 和 IL-6）也显著恢复到接近正常水平。在 Western blot 分析中，丁香脂素能显著抑制 NF-ĸB 和 iNOS 通路。
本研究表明，丁香脂素通过改善肺功能、抑制氧化应激和炎症来保护肺部免受 COPD 的侵害。
