Department of Chemistry, Tabriz Branch, Islamic Azad University, Tabriz, Iran.
Department of Chemistry, College of Science, Sultan Qaboos University, Box 36, Al-Khod 123, Muscat, Oman.
Mikrochim Acta. 2020 Nov 24;187(12):673. doi: 10.1007/s00604-020-04654-4.
Specific recognition of organophosphate pesticides (OPs) is a significant challenge for analytical researchers. Herein, surface imprinted terbium-based luminescent metal-organic framework (MOF-76) are presented as a highly specific probe for the measurement of chlorpyrifos (CP). A mesoporous molecular imprinted polymer (mMIP) layer was generated on the surface of nano-sized MOF-76 using CP, as template. The resulting mMIP-capped MOF-76 (mMIP@MOF-76) contained specific sites for adsorption of CP molecules, guaranteeing the selectivity of the designed probe. The high porosity of rod-shape MOF-76, as well as the mesoporous structure of the MIP layer improved the diffusion process and caused the high sensitivity of the probe. The detection process is based on the remarkable quenching effect of CP on the fluorescence emission of mMIP@MOF-76. Plotting the CP concentration against the fluorescence intensity (λ = 285 nm and λ = 544 nm) gave a linear curve in the concentration range 10-1000 ng mL CP, with 3.41 ng mL limit of detection. The designed probe was utilized for CP determination in fruit juice and environmental samples. The combination of the stable MOF-based support, as well as its remarkable fluorescence features and specific MIP sites, led to a highly selective and ultrasensitive detection system.Graphical abstract.
有机磷农药（OPs）的特异性识别对分析研究人员来说是一个重大挑战。在此，我们提出了基于铽的发光金属-有机骨架（MOF-76）表面印迹作为一种用于测定毒死蜱（CP）的高特异性探针。采用 CP 作为模板，在纳米尺寸的 MOF-76 表面生成介孔分子印迹聚合物（mMIP）层。所得的 mMIP 封端的 MOF-76（mMIP@MOF-76）包含了 CP 分子吸附的特异性位点，保证了设计探针的选择性。棒状 MOF-76 的高孔隙率以及 MIP 层的介孔结构改善了扩散过程，使探针具有高灵敏度。检测过程基于 CP 对 mMIP@MOF-76 的荧光发射的显著猝灭效应。绘制 CP 浓度与荧光强度（λ = 285nm 和 λ = 544nm）的关系曲线，在 10-1000ng mL CP 的浓度范围内得到了一条线性曲线，CP 的检出限为 3.41ng mL。该设计的探针用于果汁和环境样品中 CP 的测定。稳定的基于 MOF 的载体的结合，以及其显著的荧光特性和特定的 MIP 位点，导致了一种高选择性和超灵敏的检测系统。
