Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, 53714-161, Tabriz, Iran; Research Laboratory of Advanced Water and Wastewater Treatment Processes, Department of Applied Chemistry, Faculty of Chemistry, University of Tabriz, 51666-16471, Tabriz, Iran.
Electroanalytical Chemistry Laboratory, Department of Chemistry, Faculty of Science, Azarbaijan Shahid Madani University, 53714-161, Tabriz, Iran.
Talanta. 2019 Aug 15;201:286-294. doi: 10.1016/j.talanta.2019.04.023. Epub 2019 Apr 11.
Herein, high performance peroxidase-like activity of zinc and cobalt bi-metal metal-organic framework (ZnCo MOF) is reported and applied for the sensitive measurement of atropine. ZnCo MOF was synthesized by the reaction of 2-methylimidazole with Zn and Co (II) cations in aqueous media. The colorimetric and fluorometric experiments were applied to investigate the catalytic activity of obtained MOF, using o-phenylenediamine (OPD) and terephthalic acid (TA) peroxidase substrates, respectively. The results demonstrated the more efficient mimetic behavior of ZnCo MOF compared with common Zn or Co MOFs. Besides, it was found that atropine hindered the catalytic action of ZnCo MOF and this effect was intensified by increasing the atropine concentration. So, it was considered to design a sensitive analytical assay for atropine detection. To assure a high specific recognition, molecularly imprinted polymer (MIP)-based extraction using magnetic graphene oxide supports was applied to extract atropine before its determination. The combination between the high specific extraction and great catalytic activity of ZnCo MOF led to the ultrasensitive and reliable determination of atropine. The best linear range of calibration graph was achieved using fluorometric detection system for 0.1-45 ng mL atropine concentrations, and detection limit (3S/m) was 27 pg mL. The relative standard deviations (RSD %) for the determination of 1, and 10 ng mL atropine (n = 5) were 2.13% and 3.08%, respectively. The explained fluorometric assay was examined for the measurement of atropine in biological fluids (Recoveries were in the range of 95.90-103.57%), and the results were validated by an official method.
本文报道了锌钴双金属金属有机骨架（ZnCo MOF）具有优异的过氧化物酶样活性，并将其应用于灵敏检测阿托品。ZnCo MOF 通过在水相介质中 2-甲基咪唑与 Zn 和 Co（II）阳离子的反应合成。通过使用邻苯二胺（OPD）和对苯二甲酸（TA）作为过氧化物酶底物的比色和荧光实验，研究了所得 MOF 的催化活性。结果表明，与常见的 Zn 或 Co MOFs 相比，ZnCo MOF 具有更有效的模拟行为。此外，还发现阿托品抑制了 ZnCo MOF 的催化作用，并且随着阿托品浓度的增加，这种抑制作用得到了加强。因此，设计了一种用于检测阿托品的灵敏分析方法。为了确保高特异性识别，使用基于分子印迹聚合物（MIP）的磁性氧化石墨烯作为支持物，对阿托品进行萃取，然后对其进行测定。ZnCo MOF 的高特异性萃取和高催化活性的结合，实现了对阿托品的超灵敏和可靠检测。使用荧光检测系统，校准曲线的最佳线性范围为 0.1-45ngmL 阿托品浓度，检测限（3S/m）为 27pgmL。在 1 和 10ngmL 阿托品（n=5）的测定中，相对标准偏差（RSD%）分别为 2.13%和 3.08%。解释的荧光测定法用于测量生物体液中的阿托品（回收率在 95.90-103.57%之间），并通过官方方法验证了结果。
