Department of Dental Biomaterials, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Dental and Periodontal Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Pharm Nanotechnol. 2024;12(3):269-275. doi: 10.2174/2211738511666230818092706.
The aim of this study was the preparation of mesoporous silica nanoparticles co-loaded with rutin and curcumin (Rut-Cur-MSNs) and the assessment of its physicochemical properties as well as its cytotoxicity on the head and neck cancer cells (HN5). Besides, ROS generation of HN5 cells exposed to Rut-Cur-MSNs was evaluated. Several investigations showed that rutin and curcumin have potential effects as anticancer phytochemicals; however, their low aqueous solubility and poor bioavailability limited their applications. The assessment of physicochemical properties and anticancer effect of prepared nanoparticles was the objective of this study.
The physicochemical properties of produced nanoparticles were evaluated. The toxicity of Rut-Cur-MSNs on HN5 cells was assessed. In addition, the ROS production in cells treated with Rut- Cur-MSNs was assessed compared to control untreated cells.
The results showed that Rut-Cur-MSNs have mesoporous structure, nanometer size and negative surface charge. The X-ray diffraction pattern showed that the prepared nanoparticles belong to the family of silicates named MCM-41. The cytotoxicity of Rut-Cur-MSNs at 24 h was significantly higher than that of rutin-loaded MSNs (Rut-MSNs) and curcumin-loaded MSNs (Cur-MSNs) (p<0.05).
The achieved results recommend that the prepared mesoporous silica nanoparticles containing rutin and curcumin can be a useful nanoformulation for the treatment of cancer. The produced nanomaterial in this study can be helpful for cancer therapy.
本研究的目的是制备载芦丁和姜黄素的介孔硅纳米粒子（Rut-Cur-MSNs），并评估其物理化学性质及其对头颈部癌细胞（HN5）的细胞毒性。此外，还评估了暴露于 Rut-Cur-MSNs 的 HN5 细胞中 ROS 的产生。多项研究表明，芦丁和姜黄素作为抗癌植物化学物质具有潜在的作用；然而，它们的低水溶解度和差的生物利用度限制了它们的应用。本研究的目的是评估制备的纳米粒子的物理化学性质和抗癌作用。
评估了所产生的纳米粒子的物理化学性质。评估了 Rut-Cur-MSNs 对 HN5 细胞的毒性。此外，与未经处理的对照细胞相比，评估了细胞中 Rut- Cur-MSNs 产生的 ROS 。
结果表明，Rut-Cur-MSNs 具有介孔结构、纳米尺寸和负表面电荷。X 射线衍射图谱表明，所制备的纳米粒子属于 MCM-41 家族的硅酸盐。24 小时时，Rut-Cur-MSNs 的细胞毒性明显高于载芦丁的 MSNs（Rut-MSNs）和载姜黄素的 MSNs（Cur-MSNs）（p<0.05）。
研究结果表明，载芦丁和姜黄素的介孔硅纳米粒子可以作为治疗癌症的有用纳米制剂。本研究中生产的纳米材料可有助于癌症治疗。
