Silva JÚnior Joel A DA, Silva Amanda C V F DA, Figueiredo LetÍcia S, Araujo Thiago R, Freitas Israelle N, Carneiro Everardo M, Ribeiro Elane S, Ribeiro Rosane A
Universidade Federal do Rio de Janeiro, Laboratório de Fisiopatologia, Divisão de Pesquisa Integrada em Produtos Bioativos e Biociências/DPBio, Polo Novo Cavaleiros, Campus UFRJ-Macaé, Rua Alcides da Conceição, 159, 27933-378 Macaé, RJ, Brazil.
Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Biologia Estrutural e Funcional, Av. Bertrand Russel, s/n, Caixa Postal 6109, 13083-865 Campinas, SP, Brazil.
An Acad Bras Cienc. 2020 Nov 20;92(4):e20201382. doi: 10.1590/0001-3765202020201382. eCollection 2020.
D-pinitol is one of the major inositol found in plants and studies suggest its potential hypoglycemic and hypolipidemic actions in diabetic rodents. Here, we investigated the actions of D-pinitol on adiposity, and in lipid and glycemic homeostasis in monosodium glutamate (MSG)-obese mice. Swiss mice received daily subcutaneous injections of MSG [(4g/kg of body weight (BW)] or saline [1.25g/kg BW; control (CTL)] during their first five days of life. From 90-120 day-old, half of the MSG and CTL groups received 50 mg D-pinitol/kg BW/day (MPIN and CPIN groups) or vehicle (saline; MSG and CTL groups) by gavage. MSG mice displayed higher abdominal adiposity and hepatic triglycerides (TG) deposition, and increased hepatic expression of lipogenic genes (SREBP-1c, ACC-1 and FASN), but downregulation in AMPKα mRNA. MSG mice also exhibited hyperinsulinemia, islet hypersecretion and hypertrophy, glucose intolerance and insulin resistance. D-pinitol did not change adiposity, glucose intolerance, insulin resistance, but increased hepatic triglycerides (TG) content in MPIN mice, which was associated with increases in gene expressions of SREBP-1c and FASN, but reduction in AMPKα. Furthermore, D-pinitol enhanced insulin secretion in MPIN and CPIN groups. Therefore, D-pinitol enhanced glucose-induced insulin secretion, which may account to enhances hepatic lipogenesis and TG deposition in MPIN mice.
D-松醇是植物中发现的主要肌醇之一，研究表明其在糖尿病啮齿动物中具有潜在的降血糖和降血脂作用。在此，我们研究了D-松醇对谷氨酸单钠（MSG）诱导的肥胖小鼠的肥胖、脂质和血糖稳态的影响。瑞士小鼠在出生后的前五天每天皮下注射MSG[4克/千克体重（BW）]或生理盐水[1.25克/千克体重；对照组（CTL）]。从90至120日龄开始，MSG组和CTL组的一半小鼠通过灌胃接受50毫克D-松醇/千克体重/天（MPIN组和CPIN组）或溶剂（生理盐水；MSG组和CTL组）。MSG小鼠表现出更高的腹部肥胖和肝脏甘油三酯（TG）沉积，以及脂肪生成基因（SREBP-1c、ACC-1和FASN）的肝脏表达增加，但AMPKα mRNA下调。MSG小鼠还表现出高胰岛素血症、胰岛高分泌和肥大、葡萄糖不耐受和胰岛素抵抗。D-松醇没有改变肥胖、葡萄糖不耐受、胰岛素抵抗，但增加了MPIN小鼠的肝脏甘油三酯（TG）含量，这与SREBP-1c和FASN基因表达增加但AMPKα减少有关。此外，D-松醇增强了MPIN组和CPIN组的胰岛素分泌。因此，D-松醇增强了葡萄糖诱导的胰岛素分泌，这可能是导致MPIN小鼠肝脏脂肪生成和TG沉积增加的原因。
