Department of Cardiovascular and Metabolic Sciences, Cleveland Clinic, Cleveland, OH, USA.
Department of Psychiatry and Biobehavioral Sciences, Semel Institute for Neuroscience and Human Behavior, University of California Los Angeles, Los Angeles, CA, USA.
Methods Mol Biol. 2021;2236:157-175. doi: 10.1007/978-1-0716-1060-2_13.
We will first describe analysis of MDSC subsets from patient tumors with multicolor flow cytometry. The key components of this methodology are to obtain viable single cell suspensions and eliminate red blood cell contamination.
我们将首先描述用多色流式细胞术分析患者肿瘤中的 MDSC 亚群。该方法学的关键组成部分是获得活的单细胞悬浮液并消除红细胞污染。
