Ditommaso Savina, Giacomuzzi Monica, Memoli Gabriele, Garlasco Jacopo, Zotti Carla M
Department of Public Health and Pediatrics, University of Turin, 10126 Torino, Italy.
Pathogens. 2020 Nov 23;9(11):978. doi: 10.3390/pathogens9110978.
Evidence to date indicates that heater-cooler units (HCUs) and heater units (HUs) can generate potentially infectious aerosols containing a range of opportunistic pathogens such as , other non-tuberculous mycobacterial (NTM) species, and spp. Our purpose was to determine the extent of contamination and total viable count (TVC) in HCUs and HUs and to analyze the relationship by water system design of devices of two different brands (LivaNova vs. Maquet).
spp. were detected and quantified by our optimized PMA-qPCR protocol; TVCs were assessed according to ISO protocol 6222. Analyses were performed in the first sampling round and after six months of surveillance.
Overall, spp. was detected in 65.7% of devices. In the second sampling round, positivity rates were significantly lower in water samples from the Maquet devices compared to the LivaNova ones (27.3% vs. 61.5%). LivaNova HCUs also yielded more , and aquatic bacteria counts than Maquet in both first and second-round samples.
We recommend that all surgical patients and staff exposed to aerosols from thermoregulatory devices should be followed up for infection and that microbiological surveillance on such devices should be conducted regularly as precautionary principle.
迄今为止的证据表明，加热 - 冷却装置（HCU）和加热装置（HU）可产生含有一系列机会性病原体的潜在传染性气溶胶，如 、其他非结核分枝杆菌（NTM）菌种以及 属菌种。我们的目的是确定HCU和HU中的 污染程度和总活菌数（TVC），并通过两种不同品牌（LivaNova与Maquet）设备的水系统设计分析两者之间的关系。
通过我们优化的PMA - qPCR方案检测和定量 属菌种；根据ISO协议6222评估TVC。在第一轮采样和监测六个月后进行分析。
总体而言，65.7%的设备检测到 属菌种。在第二轮采样中，与LivaNova设备相比，Maquet设备水样中的 阳性率显著更低（27.3%对61.5%）。在第一轮和第二轮样本中，LivaNova的HCU产生的 、水生细菌数量也比Maquet更多。
我们建议，应对所有暴露于温度调节设备气溶胶的手术患者和工作人员进行 感染随访，并应按照预防原则定期对此类设备进行微生物监测。
