Alvi Mughees Aizaz, Ohiolei John Asekhaen, Saqib Muhammad, Tayyab Muhammad Haleem, Zafar Khan Muhammad Umar, Li Li, Aqib Amjad Islam, Hassan Ali, Alvi Anum Aizaz, Qamar Warda, Fu Bao-Quan, Yan Hong-Bin, Jia Wan-Zhong
State Key Laboratory of Veterinary Etiological Biology, National Professional Laboratory for Animal Echinococcosis, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.
Front Vet Sci. 2020 Nov 10;7:594599. doi: 10.3389/fvets.2020.594599. eCollection 2020.
is the larval stage of commonly found in the brain (cerebral form), intramuscular and subcutaneous tissues (non-cerebral form) of ungulates. Globally, few reports exist on the molecular characterization and genetic diversity of . with none available for Pakistan. The current study molecularly characterized 12 . isolates surgically recovered from sheep ( = 4) and goats ( = 8) from a total of 3,040 small ruminants using a portion of the cytochrome oxidase subunit 1 (1) mitochondrial () gene. NCBI BLAST search confirmed the identity of each isolate. A high haplotype and a low nucleotide diversity with three haplotypes from the 12 isolates were observed. The findings suggest the existence of unique haplotypes of in Pakistan. The negative value of Tajima's and the positive value of Fu's Fs were inconsistent with population expansion, however, the sample size was small. Bayesian phylogeny revealed that all Pakistani isolates alongside the Chinese sequences (obtained from GenBank) constituted a cluster while sequences from other regions constituted another cluster. This is the first molecular study to determine the genetic diversity of . in Pakistan and serves as a foundation for prospective studies on the prevalence and population structure of in the country. Furthermore, in this study, we amplified only a partial segment of the 1 gene from a limited sample size. This could have implications on the interpretation of the actual population structure in reality. Thus, we recommend future studies to consider a larger sample size in a massive epidemiological survey for further insights.
是偶蹄目动物常见于大脑（脑型）、肌肉和皮下组织（非脑型）中的幼虫阶段。在全球范围内，关于其分子特征和遗传多样性的报道很少。在巴基斯坦则尚无相关报道。本研究对从总共3040只小反刍动物中通过手术回收的12个分离株进行了分子特征分析，这些分离株来自绵羊（n = 4）和山羊（n = 8），使用了细胞色素c氧化酶亚基1（CO1）线粒体（mt）基因的一部分。NCBI BLAST搜索确认了每个分离株的身份。观察到高单倍型和低核苷酸多样性，12个分离株中有三种单倍型。研究结果表明巴基斯坦存在独特的单倍型。 Tajima's D的负值和Fu's Fs的正值与种群扩张不一致，然而，样本量较小。贝叶斯系统发育分析表明，所有巴基斯坦分离株与中国序列（从GenBank获得）构成一个簇，而其他地区的序列构成另一个簇。这是第一项确定巴基斯坦该物种遗传多样性的分子研究，为该国该物种的流行率和种群结构的前瞻性研究奠定了基础。此外，在本研究中，我们仅从有限的样本量中扩增了CO1基因的部分片段。这可能会对实际种群结构的解释产生影响。因此，我们建议未来的研究在大规模流行病学调查中考虑更大的样本量，以获得更深入的见解。
