Al Malki Jamila S, Hussien Nahed Ahmed
Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Open Life Sci. 2021 Nov 27;16(1):1252-1260. doi: 10.1515/biol-2021-0131. eCollection 2021.
Taeniids, consisting of two genera and , are obligatory tapeworms of mammals, and their pathogenicity was due to infection with larval stages. Hydatid (the larval stage of ) and coenurus (the larval stage of ) cysts are prevalent in domestic, wild ruminants, livestock, swine, and dogs, and accidentally they could also be found in humans. They lead to different clinical manifestations that cause economic loss in livestock and human morbidity. In Saudi Arabia, few studies were performed on hydatid and coenurus cyst genetic variations. The main goal of the present study was to identify and cyst isolates collected from slaughtered Harri sheep in Saudi Arabia by partial sequencing with PCR amplification of the cytochrome C oxidase 1 (1) gene. Molecular and phylogenetic evaluation based on 1 sequences indicated that cyst isolates belong to and , respectively, successfully submitted in NCBI Genbank. Molecular characterization showed a low nucleotide diversity with two submitted isolates of coenurus with related isolates of Genbank. Conversely, isolates showed higher nucleotide diversity. The reported data could serve as a foundation for future molecular epidemiological and biological studies.
带绦虫由两个属组成，是哺乳动物的专性绦虫，其致病性源于幼虫阶段的感染。棘球蚴（的幼虫阶段）和多头蚴（的幼虫阶段）囊肿在家养和野生反刍动物、家畜、猪和狗中普遍存在，偶尔也会在人类中发现。它们会导致不同的临床表现，给家畜造成经济损失并导致人类发病。在沙特阿拉伯，对棘球蚴和多头蚴囊肿的遗传变异进行的研究很少。本研究的主要目标是通过对细胞色素C氧化酶1（CO1）基因进行PCR扩增并部分测序，鉴定从沙特阿拉伯屠宰的哈里羊中收集的棘球蚴和多头蚴囊肿分离株。基于CO1序列的分子和系统发育评估表明，囊肿分离株分别属于和，并已成功提交至NCBI基因库。分子特征显示，提交的两个多头蚴分离株与基因库中的相关分离株相比，核苷酸多样性较低。相反，棘球蚴分离株显示出较高的核苷酸多样性。报告的数据可为未来的分子流行病学和生物学研究奠定基础。
