Sanvitto G L, Azambuja N A, Marques M
Departamento de Fisiologia, Farmacologia e Biofísica, Universidade Federal do Rio Grande do Sul, Porto Alergre, RS, Brasil.
Physiol Behav. 1987;41(5):523-4. doi: 10.1016/0031-9384(87)90093-x.
The difficulty in obtaining cerebrospinal fluid (CFS) in an efficient and simple manner in rats prompts us to introduce a new technique that makes use of a cannula placed in the lateral ventricle. The cannula is implanted with a stereotaxic apparatus and the CSF is collected with a glass capillary tube. The technique has proved to work well for experiments in which the CSF must be free of blood. It also permits the collection of volumes of CSF sufficient for radioimmunoassays, and may be used in chronic experiments.
在大鼠中高效且简便地获取脑脊液（CFS）存在困难，这促使我们引入一种新技术，该技术利用置于侧脑室的套管。通过立体定位仪植入套管，并用玻璃毛细管收集脑脊液。事实证明，该技术在脑脊液必须无血的实验中效果良好。它还能收集足以用于放射免疫测定的脑脊液量，并且可用于慢性实验。
