Frankfurt Institute for Advanced Studies, Frankfurt am Main, Germany.
Ernst Strüngmann Institute (ESI) for Neuroscience in cooperation with Max Planck Society, Frankfurt am Main, Germany.
Elife. 2020 Nov 26;9:e60920. doi: 10.7554/eLife.60920.
Class I ventral posterior dendritic arborisation (c1vpda) proprioceptive sensory neurons respond to contractions in the larval body wall during crawling. Their dendritic branches run along the direction of contraction, possibly a functional requirement to maximise membrane curvature during crawling contractions. Although the molecular machinery of dendritic patterning in c1vpda has been extensively studied, the process leading to the precise elaboration of their comb-like shapes remains elusive. Here, to link dendrite shape with its proprioceptive role, we performed long-term, non-invasive, in vivo time-lapse imaging of c1vpda embryonic and larval morphogenesis to reveal a sequence of differentiation stages. We combined computer models and dendritic branch dynamics tracking to propose that distinct sequential phases of stochastic growth and retraction achieve efficient dendritic trees both in terms of wire and function. Our study shows how dendrite growth balances structure-function requirements, shedding new light on general principles of self-organisation in functionally specialised dendrites.
I 类腹后树突分支（c1vpda）本体感受神经元在爬行时对幼虫体壁的收缩做出反应。它们的树突分支沿着收缩的方向延伸，这可能是在爬行收缩过程中最大限度地增加膜曲率的功能要求。尽管 c1vpda 中树突模式形成的分子机制已经得到了广泛的研究，但导致其梳状形状精确细化的过程仍然难以捉摸。在这里，为了将树突形状与其本体感受作用联系起来，我们对 c1vpda 胚胎和幼虫形态发生进行了长期、非侵入性、体内延时成像，以揭示一系列分化阶段。我们结合计算机模型和树突分支动力学跟踪，提出了随机生长和回缩的不同顺序阶段如何以线和功能的方式实现有效的树突。我们的研究表明了树突生长如何平衡结构-功能要求，为功能特化树突中的自组织一般原则提供了新的见解。
