Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA; Department of Pathology, Massachusetts General Hospital, Boston, MA, USA; Harvard Medical School, Department of Medicine, Boston, MA, USA.
Department of Pathology, Massachusetts General Hospital, Boston, MA, USA.
Diagn Microbiol Infect Dis. 2021 Feb;99(2):115210. doi: 10.1016/j.diagmicrobio.2020.115210. Epub 2020 Sep 19.
Surveillance for antibiotic-resistant (AR) bacteria is challenging. We evaluated AR Enterobacterales survival in stool over various transport conditions. Stool in Cary-Blair medium was spiked with AR Enterobacterales, held at 3 °C, 20 °C, or 37 °C, and cultured on days 3, 8, and 15. Stool from US international travelers sent through the US mail was also screened. We compared recovery rates using Fisher's exact tests and linear regression models. AR Enterobacterales recovery reduced with time (86% versus 75% versus 61% at days 3, 8, and 15; Beta for linear trend=-0.02, r=0.99, P=0.02) and colder temperatures [56% (3 °C) versus 89% (20 °C) versus 86% (37 °C); P=0.003]. Traveler sample recovery also reduced with transport time (Beta for linear trend=-0.03, r=0.70, P=0.01) but not with season [20% (cold) versus 22% (warm), P=0.7]. AR Enterobacterales are found over variable transport conditions, providing rationale for expanding surveillance sample processing timelines.
抗生素耐药（AR）细菌的监测具有挑战性。我们评估了在不同运输条件下粪便中 AR 肠杆菌科细菌的存活情况。在 Cary-Blair 培养基中加入 AR 肠杆菌科细菌，将其置于 3°C、20°C 或 37°C 下，在第 3、8 和 15 天进行培养。还对通过美国邮件发送的美国国际旅行者的粪便进行了筛查。我们使用 Fisher 精确检验和线性回归模型比较了回收率。随着时间的推移，AR 肠杆菌科细菌的回收率降低（第 3、8 和 15 天分别为 86%、75%和 61%；线性趋势的 Beta 值为-0.02，r=0.99，P=0.02），低温下的回收率也降低[56%（3°C）、89%（20°C）和 86%（37°C）；P=0.003]。旅行者样本的回收率也随运输时间的延长而降低（线性趋势的 Beta 值为-0.03，r=0.70，P=0.01），但与季节无关[20%（冷季）和 22%（暖季），P=0.7]。AR 肠杆菌科细菌在不同的运输条件下均有发现，这为扩大监测样本处理时间线提供了依据。
