College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; National Marine Environmental Monitoring Center, Dalian 116023, China; College of Ecology and Environment, Hainan Tropical Ocean University, Sanya 572022, China; College of Marine Technology and Environment, Dalian Ocean University, Dalian 116023, China.
College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China; National Marine Environmental Monitoring Center, Dalian 116023, China.
Mar Pollut Bull. 2021 Jan;162:111829. doi: 10.1016/j.marpolbul.2020.111829. Epub 2020 Nov 23.
Quantifying the occurrence of Antarctic antibiotic-resistant bacteria (ARB) is essential for assessing the level of pollution and assessing the "baseline" or background level of ARB in human uninhabited environments. Animal feces, soil, and sediments were sampled from Fildes Peninsula. The abundance of sulfamethazine- and ciprofloxacin-resistance bacteria and antibotic resistance genes (ARGs) within ARB were investigated. The results showed Ciprofloxacin- and Sulfamethazine-resistant bacteria isolated from samples accounted for the highest abundances of 30 CFU/g and 79.8 CFU/g, respectively. The dominant genus of Sulfamethazine-and quinolone-resistance bacteria was Pseudomonas and Arthrobacter, respectively. 106 ARGs were detected from ARB. Strong positive correlations between mobile genetic elements (MGEs) and ARGs were found, what is relatively novel observation that the mechanism is confirmed to also occur in the Antarctic. This study reveals the compositional characteristics of ARGs of strains in Antarctic, providing support for the source of Antarctic antibiotic resistance and drug resistance mechanisms.
量化南极地区抗生素耐药细菌 (ARB) 的发生情况对于评估污染程度以及评估人类无人居住环境中 ARB 的“基线”或背景水平至关重要。本研究从菲尔德斯半岛采集了动物粪便、土壤和沉积物样本。研究了 ARB 中磺胺甲恶唑和环丙沙星耐药细菌和抗生素耐药基因 (ARGs) 的丰度。结果表明，从样品中分离出的环丙沙星和磺胺甲恶唑耐药细菌分别占 30 CFU/g 和 79.8 CFU/g 的最高丰度。磺胺甲恶唑和喹诺酮类耐药细菌的主要属分别为假单胞菌和节杆菌。从 ARB 中检测到 106 个 ARGs。发现移动遗传元件 (MGEs) 和 ARGs 之间存在强烈的正相关，这是一个相对新颖的观察结果，证实该机制也发生在南极。本研究揭示了南极地区菌株中 ARGs 的组成特征，为南极地区抗生素耐药性的来源和耐药机制提供了支持。
