Huang Jiangqing, Zhang Shengcen, Zhang Shuyu, Zhao Zhichang, Cao Yingping, Chen Min, Li Bin
Department of Clinical Laboratory, Fujian Medical University Union Hospital, Fuzhou, Fujian 350001, People's Republic of China.
Department of Laboratory Medicine, Fujian Medical University, Fuzhou, Fujian 350001, People's Republic of China.
Infect Drug Resist. 2020 Nov 20;13:4167-4175. doi: 10.2147/IDR.S277681. eCollection 2020.
Sequence type 1193 is a new such lineage among fluoroquinolone-resistant , which has risen dramatically within the last several years. However, reasons for rapid emergence and successful spread of ST1193 remain unclear. The aim of this study was to compare the pathogenicity and survivability features of ST1193 with global epidemic lineage, ST131.
A total of 30 were used in this study. Isolates were divided into two groups, ST1193 (n=15) and ST131 (n=15). Adhesion and invasion to T24 cells and resistance to serum were quantified and compared among two groups. Biofilm formation capacity was assessed by crystal violet assay. Macrocolony formation was assessed on macrocolony formation plates. Resistance to hydrogen peroxide was performed by broth microdilution. RAW264.7 cells were used to assess the anti-phagocytic function of different isolates.
Adhesion and invasion assays revealed that ST1193 could adhere and invade T24 cells ( <0.05). 93.3% of ST1193 could form biofilms. The majority of ST1193 (66.7%) possessed no curli/no cellulose on macrocolony formation plates. ST1193 showed significant growth in serum and hydrogen peroxide and illustrated higher anti-phagocytic function to RAW264.7 cells ( <0.05). Group analysis showed that ST1193 was similar to ST131 in pathogenicity- and survivability-associated phenotypic characteristics ( >0.05).
Our study provided more insights into pathogenicity and survivability features of ST1193, which was similar to ST131. Our study could be of great importance in understanding the emergence of global spread ST1193. Strategic and continued surveillance should be carried out to prevent the infections caused by ST1193.
1193型序列是耐氟喹诺酮类中的一种新谱系，在过去几年中急剧增加。然而，ST1193快速出现并成功传播的原因仍不清楚。本研究的目的是比较ST1193与全球流行谱系ST131的致病性和生存能力特征。
本研究共使用了30株菌株。分离株分为两组，ST1193（n = 15）和ST131（n = 15）。对两组之间对T24细胞的黏附与侵袭以及对血清的抗性进行定量和比较。通过结晶紫测定法评估生物膜形成能力。在大菌落形成平板上评估大菌落形成情况。通过肉汤微量稀释法检测对过氧化氢的抗性。使用RAW264.7细胞评估不同分离株的抗吞噬功能。
黏附与侵袭试验显示，ST1193能够黏附并侵袭T24细胞（P<0.05）。93.3%的ST1193能够形成生物膜。在大菌落形成平板上，大多数ST1193（66.7%）不具有卷曲菌毛/不产生纤维素。ST1193在血清和过氧化氢中显示出显著生长，并对RAW264.7细胞表现出更高的抗吞噬功能（P<0.05）。分组分析表明，ST1193在与致病性和生存能力相关的表型特征方面与ST131相似（P>0.05）。
我们的研究为ST1193的致病性和生存能力特征提供了更多见解，其与ST131相似。我们的研究对于理解全球传播的ST1193的出现可能具有重要意义。应进行战略性持续监测以预防由ST1193引起的感染。
