人膀胱移行细胞癌中运铁蛋白受体的表达
Transferrin receptor expression by human bladder transitional cell carcinomas.
作者信息
Seymour G J, Walsh M D, Lavin M F, Strutton G, Gardiner R A
机构信息
Department of Surgery, University of Queensland, St. Lucia, Brisbane, Australia.
出版信息
Urol Res. 1987;15(6):341-4. doi: 10.1007/BF00265663.
The expression of transferrin receptors (TFR) by normal and neoplastic urothelial cells was studied in "control" patients and in patients with transitional cell carcinoma of the bladder. These tumours were graded independently and consisted of 19 grade I, 30 grade II and 19 grade III lesions. TFRs were identified using a monoclonal antibody specific for TFR (OKT9) in an immunofluorescent or avidin/biotin-immunoperoxidase technique on fresh frozen sections. TFRs were not detected on normal urothelium. However, positive staining was found to increase with increasing pathological grade and stage of the tumours, ranging from 31.6% of grade I to 78.9% of grade III tumours and 51.2% of pTa (mucosa only lesions) to 87.5% of pT2/pT2+ (muscle invasion +/- deeper) primary urothelial malignancies.
在“对照”患者和膀胱移行细胞癌患者中，研究了正常和肿瘤性尿路上皮细胞中转铁蛋白受体（TFR）的表达情况。这些肿瘤进行了独立分级，包括19例I级、30例II级和19例III级病变。使用针对TFR的单克隆抗体（OKT9），通过免疫荧光或抗生物素蛋白/生物素-免疫过氧化物酶技术，在新鲜冰冻切片上鉴定TFR。在正常尿路上皮中未检测到TFR。然而，发现阳性染色随着肿瘤病理分级和分期的增加而增加，范围从I级肿瘤的31.6%到III级肿瘤的78.9%，以及pTa（仅黏膜病变）的51.2%到pT2/pT2 +（肌肉浸润+/-更深）原发性尿路上皮恶性肿瘤的87.5%。
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