CERMN, UNICAEN Université de Caen Normandie, F-14000 Caen, France.
Mol Pharm. 2021 Jan 4;18(1):44-64. doi: 10.1021/acs.molpharmaceut.0c00857. Epub 2020 Nov 27.
Surface functionalization of lipid-based nanocarriers (LBNCs) with targeting ligands has attracted huge interest in the field of nanomedicines for their ability to overcome some physiological barriers and their potential to deliver an active molecule to a specific target without causing damage to healthy tissues. The principal objective of this review is to summarize the present knowledge on LBNC decoration used for biomedical applications, with an emphasis on the ligands used, the functionalization approaches, and the purification methods after ligand corona formation. The most potent experimental techniques for the LBNC surface characterization are described. The potential of promising methods such as nuclear magnetic resonance spectroscopy and isothermal titration calorimetry to characterize ligand surface corona is also outlined.
基于脂质的纳米载体（LBNC）的表面功能化与靶向配体的结合，引起了纳米医学领域的极大兴趣，因为它能够克服一些生理障碍，并有可能将活性分子递送到特定的靶标而不会对健康组织造成损害。本篇综述的主要目的是总结目前用于生物医学应用的 LBNC 修饰知识，重点介绍所使用的配体、功能化方法以及配体冠形成后的纯化方法。描述了用于 LBNC 表面特性描述的最有效实验技术。还概述了诸如核磁共振波谱和等温滴定量热法等有前途的方法在表征配体表面冠方面的潜力。
