School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China.
Food Funct. 2020 Dec 1;11(12):10876-10885. doi: 10.1039/d0fo02575e. Epub 2020 Nov 27.
A sulfated polysaccharide extracted from Gracilaria lemaneiformis (GLP) with a prominent effect in regulating lipid metabolism was isolated. The molecular weight was 31.5 kDa and it was composed mainly of galactose, glucose and xylose. Fourier-transform infrared (FT-IR) spectrum and nuclear magnetic resonance (NMR) analysis suggested that GLP was composed of the following repeating unit: [3-β-Gal-4(OSO3)-1→4-α-3,6-anhydrogal-2(OSO3)-1→]. GLP could significantly decrease serum total cholesterol, triglyceride and free fatty acid levels and lower alanine aminotransferase and aspartate aminotransferase activities in high-fat-diet mice. Additionally, GLP could keep the body weight and attenuate accumulation of fat surrounding the liver and epididymis induced by high-fat diet. Results of RT-PCR indicated that GLP might regulate lipid metabolism and accelerate free fatty acid oxidation by up-regulating the expression of the PPARα, ACS and CPT1a gene. The present study suggests that GLP may be potentially useful for regulating lipid metabolism.
从江蓠（Gracilaria lemaneiformis）中提取到一种具有显著调节脂质代谢作用的硫酸多糖（GLP）。其分子量为 31.5 kDa，主要由半乳糖、葡萄糖和木糖组成。傅里叶变换红外（FT-IR）光谱和核磁共振（NMR）分析表明，GLP 由以下重复单元组成：[3-β-Gal-4(OSO3)-1→4-α-3,6-anhydrogal-2(OSO3)-1→]。GLP 可显著降低高脂饮食小鼠的血清总胆固醇、甘油三酯和游离脂肪酸水平，并降低丙氨酸转氨酶和天冬氨酸转氨酶活性。此外，GLP 可保持体重，减轻高脂饮食引起的肝脏和附睾周围脂肪堆积。RT-PCR 结果表明，GLP 可能通过上调 PPARα、ACS 和 CPT1a 基因的表达来调节脂质代谢和加速游离脂肪酸氧化。本研究提示 GLP 可能具有调节脂质代谢的潜力。
