G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Pushchino Center for Biological Research of the Russian Academy of Sciences, prosp. Nauki 5, Pushchino, Moscow Region, 142290, Russia.
Belozersky Research Institute of Physico-Chemical Biology, MSU, Leninskiye Gory, Moscow, 119899, Russia.
Folia Microbiol (Praha). 2021 Apr;66(2):247-253. doi: 10.1007/s12223-020-00840-2. Epub 2020 Nov 27.
The yeast Candida maltosa is a model organism for studying adaptive changes in the structure and function of the cell wall when consuming water-insoluble nutrient sources. The cells of C. maltosa that utilize hydrocarbons contain supramolecular structures, so-called "canals" in the cell wall. Differences in protein profiles of culture liquids and cell wall extracts of C. maltosa grown on glucose and hexadecane were analyzed. Three proteins specific of cells grown on hexadecane were revealed using mass spectrometry: glycosyl hydrolase EPD2 in the culture liquid; a protein belonging to the cytochrome C family in the 0.5 mol/L NaCl extract; and PPIA_CANAL protein known as chaperone, in the 0.1% SDS extract. The possible role of these proteins in cell wall structures responsible for adaptation to hexadecane utilization is discussed.
酿酒酵母是研究细胞在利用水不溶性营养源时细胞壁结构和功能适应性变化的模式生物。利用烃类物质的酿酒酵母细胞的细胞壁中含有超分子结构，即所谓的“通道”。分析了在葡萄糖和十六烷上生长的酿酒酵母培养液和细胞壁提取物的蛋白质图谱。利用质谱法揭示了在十六烷上生长的细胞特有的三种蛋白质：培养液中的糖苷水解酶 EPD2；0.5mol/L NaCl 提取物中属于细胞色素 C 家族的一种蛋白质；0.1%SDS 提取物中的 PPIA_CANAL 蛋白，又称伴侣蛋白，它与细胞适应十六烷的利用有关。讨论了这些蛋白质在负责适应十六烷利用的细胞壁结构中的可能作用。
