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Oxygen limitation induces indirectly the synthesis of cytochrome P-450 mRNA in alkane-growing Candida maltosa.

作者信息

Wiedmann B, Wiedmann M, Mauersberger S, Schunck W H, Müller H G

机构信息

Central Institute of Molecular Biology, Academy of Sciences of the GDR, Berlin-Buch.

出版信息

Biochem Biophys Res Commun. 1988 Jan 29;150(2):859-65. doi: 10.1016/0006-291x(88)90471-8.

DOI:10.1016/0006-291x(88)90471-8
PMID:3342052
Abstract

Candida maltosa cells grown on hexadecane under oxygen limitation have an up to 6-fold higher cytochrome P-450 content in comparison to cells cultivated at oxygen saturation. We show by mRNA quantification using an in vitro translation system and subsequent specific immunoprecipitation that the cytochrome P-450 induction occurs mainly on the transcriptional level. The signal for induction may be the enhanced intracellular hexadecane concentration owing to a reduced hydroxylation capacity of the cytochrome P-450 at oxygen limitation.

摘要

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