Wiedmann B, Wiedmann M, Kärgel E, Schunck W H, Müller H G
Biochem Biophys Res Commun. 1986 May 14;136(3):1148-54. doi: 10.1016/0006-291x(86)90454-7.
In the yeast Candida maltosa the level of cytochrome P-450 was 100-300-fold higher in alkane-grown cells than in glucose-grown ones (detected by a radioimmunoassay). It was shown immunochemically (1) that this was not the result of an assembly of preexisting apoenzyme with the prosthetic heme group. By cell-free translation of total poly(A)RNA in a wheat germ system and subsequent immunoprecipitation it was shown that the amount of mRNA coding for cytochrome P-450 paralleled its concentration in the cell.
在麦芽糖假丝酵母中，烷烃培养的细胞中细胞色素P - 450的水平比葡萄糖培养的细胞高100 - 300倍（通过放射免疫测定法检测）。免疫化学分析表明（1），这并非预先存在的脱辅基酶与辅基血红素基团组装的结果。通过在小麦胚芽系统中对总聚腺苷酸RNA进行无细胞翻译并随后进行免疫沉淀，结果表明编码细胞色素P - 450的mRNA量与其在细胞中的浓度平行。
