Bursa Technical University, Department of Chemistry, 16310, Bursa, Turkey; Gebze Technical University, Department of Chemistry, 41400, Gebze, Kocaeli, Turkey.
Gebze Technical University, Department of Chemistry, 41400, Gebze, Kocaeli, Turkey.
Food Chem Toxicol. 2021 Jan;147:111886. doi: 10.1016/j.fct.2020.111886. Epub 2020 Nov 25.
The present work describes the first synthesis of novel asymmetric zinc (II) phthalocyanine (ZnPc) including three boron dipyrromethene (BODIPY) and one ethyloxy azido moieties. Moreover, single walled carbon nanotube (SWCNT) surface was functionalized by this ZnPc containing BODIPY; using the azide-alkyne Huisgen cycloaddition (Click) reaction to obtain SWCNT-ZnPc hybrid material. Structural, thermal and morphological characterizations of both ZnPc and SWCNT-ZnPc hybrid were carried out in-depth by spectroscopic, thermal and microscopic techniques. In this study, the synthesized SWCNT-ZnPc material was decorated on composite glassy carbon electrode (GCE) by means of an easy and a practical drop cast method. The modified electrode was tested as a non-enzymatic electrochemical sensor in various common pesticides such as methyl parathion, deltamethrin, chlorpyrifos and spinosad. Electrochemical behavior of non-enzymatic electrode (GCE/SWCNT-ZnPc) was determined via cyclic voltammetry and differential pulse voltammetry. The non-enzymatic sensor demonstrated high selectivity for methyl parathion in a wide linear range (2.45 nM-4.0 × 10 M), low limit of detection value (1.49 nM) and high sensitivity (0.1847 μA nM). Also, the developing non-enzymatic sensor exhibited good repeatability (RSD = 2.3% for 10 electrodes) and stability (85.30% for 30 days). Validation guidelines by HPLC and statistical analysis showed that the proposed voltammetric method were precise, accurate, sensitive, and can be used for the routine quality control of methyl parathion determination in juice samples.
本工作首次合成了新型不对称锌（II）酞菁（ZnPc），其中包含三个硼二吡咯亚甲基（BODIPY）和一个乙氧基叠氮基团。此外，通过该含 BODIPY 的 ZnPc 将单壁碳纳米管（SWCNT）表面功能化；使用叠氮-炔基 Huisgen 环加成（Click）反应获得 SWCNT-ZnPc 杂化材料。通过光谱、热和显微镜技术对 ZnPc 和 SWCNT-ZnPc 杂化材料进行了深入的结构、热和形态表征。在本研究中，通过简单实用的滴铸法将合成的 SWCNT-ZnPc 材料修饰在复合玻碳电极（GCE）上。修饰后的电极作为非酶电化学传感器，在各种常见的杀虫剂如甲基对硫磷、溴氰菊酯、毒死蜱和多杀菌素中进行了测试。通过循环伏安法和差分脉冲伏安法测定非酶电极（GCE/SWCNT-ZnPc）的电化学行为。非酶传感器对甲基对硫磷具有高选择性，线性范围宽（2.45 nM-4.0×10-6 M），检测限低（1.49 nM），灵敏度高（0.1847 μA nM）。此外，所开发的非酶传感器表现出良好的重复性（10 个电极的 RSD=2.3%）和稳定性（30 天的 85.30%）。HPLC 验证指南和统计分析表明，所提出的伏安法精确、准确、灵敏，可用于果汁样品中甲基对硫磷常规质量控制。
