采用固相酶免疫分析法测定精浆、唾液和血清中的睾酮。

Measurement of testosterone in seminal plasma, saliva and serum by solid-phase enzymeimmunoassay.

作者信息

Shieh C C, Chang S C, Tzeng C R, Huang J J, Nir W J, Hong C Y

机构信息

Department of Medicine, Veterans General Hospital, Taipei, Taiwan R.O.C.

出版信息

Andrologia. 1987 Nov-Dec;19(6):614-9. doi: 10.1111/j.1439-0272.1987.tb01909.x.

DOI:10.1111/j.1439-0272.1987.tb01909.x

PMID:3324829

Abstract

When testosterone concentrations in serum, saliva and seminal plasma were measured with a solid-phase enzymeimmunoassay in which antitestosterone antibody was bound to CNBr-Sepharose-4B, we found the ratios of testosterone in saliva and seminal plasma to that in serum were 2.5 +/- 0.2% and 5.19 +/- 0.42%. Testosterone level in saliva could be an index of free serum testosterone. In addition, we found a correlation between sperm motility and testosterone level in seminal plasma when sperm motility was measured with a transmembrane migration method. This enzymeimmunoassay could be applied to clinical studies of testosterone.

摘要

当使用抗睾酮抗体结合到溴化氰活化的琼脂糖4B上的固相酶免疫分析法测量血清、唾液和精浆中的睾酮浓度时，我们发现唾液和精浆中睾酮与血清中睾酮的比率分别为2.5±0.2%和5.19±0.42%。唾液中的睾酮水平可能是游离血清睾酮的一个指标。此外，当用跨膜迁移法测量精子活力时，我们发现精子活力与精浆中的睾酮水平之间存在相关性。这种酶免疫分析法可应用于睾酮的临床研究。