研究报告：鸭瘟病毒糖蛋白 I 影响细胞间传播和最终包膜获得。

Research Note: Duck plague virus glycoprotein I influences cell-cell spread and final envelope acquisition.

机构信息

Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan 611130, P.R. China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu City, Sichuan 611130, P.R. China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu City, Sichuan 611130, P.R. China.

出版信息

Poult Sci. 2020 Dec;99(12):6647-6652. doi: 10.1016/j.psj.2020.08.065. Epub 2020 Sep 15.

DOI:10.1016/j.psj.2020.08.065

PMID:33248580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7704973/

Abstract

To determine the role of glycoprotein I (gI) in duck plague virus (DPV), a gI-deleted mutant (BAC-CHv-ΔgI) and a gI-revertant virus (BAC-CHv-ΔgI Rev) were constructed by using a markerless two-step Red recombination system implemented on the DPV genome cloned into a bacterial artificial chromosome (BAC). Mutants were characterized on duck embryo fibroblast (DEF) cells compared with wild-type virus. BAC-CHv-ΔgI produced viral plaques on DEF cells that were on average approximately 57.2% smaller than those produced by BAC-CHv-ΔgI Rev and wild-type virus. Electron microscopy confirmed that deleting of gI resulted in nucleocapsids accumulated around the cytoplasm vesicles and few of them could complete the final envelopment process. These results clearly indicated that DPV gI plays significant roles in viral cell-cell spread and viral final envelopment process.

摘要

为了确定糖蛋白 I（gI）在鸭瘟病毒（DPV）中的作用，使用无标记的两步 Red 重组系统构建了 gI 缺失突变体（BAC-CHv-ΔgI）和 gI 回复病毒（BAC-CHv-ΔgI Rev），该系统在 DPV 基因组克隆到细菌人工染色体（BAC）上实施。与野生型病毒相比，在鸭胚成纤维细胞（DEF）上对突变体进行了表征。BAC-CHv-ΔgI 在 DEF 细胞上产生的病毒斑平均比 BAC-CHv-ΔgI Rev 和野生型病毒小约 57.2%。电子显微镜证实，gI 的缺失导致核衣壳在细胞质囊泡周围积累，其中很少有能够完成最终包膜过程。这些结果清楚地表明，DPV gI 在病毒细胞间传播和病毒最终包膜过程中发挥重要作用。