鸭瘟病毒囊膜蛋白 vp22 在二次包膜和细胞间传播中起关键作用。

Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread.

机构信息

Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu City, 611130, Sichuan, China.

Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Wenjiang, Chengdu City, 611130, Sichuan, China.

出版信息

Vet Res. 2023 Jul 17;54(1):60. doi: 10.1186/s13567-023-01191-9.

DOI:10.1186/s13567-023-01191-9

PMID:37461115

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10351120/

Abstract

Duck plague virus (DPV) is one of the major infectious and fatal diseases of geese, ducks, and other wild waterfowl. The DPV UL49 gene product VP22 is one of the most abundant tegument proteins. However, the role of the DPV VP22 is enigmatic to be clarified. In this study, we found deletion of the UL49 gene resulted in reduced viral growth curve and smaller plaque size in duck embryo fibroblast (DEF) cells, confirming that DPV VP22 is required for efficient viral growth in vitro. In addition, deletion of the UL49 gene inhibited the secondary envelopment of the virus, the release of viral particles, and the spread of viruses between cells. Our study signified the importance of VP22 for DPV secondary envelopment, release, cell-to-cell spread, and accumulation of viral RNA. These findings provide a basis for further study of the function of VP22 in DPV or other herpesviruses.

摘要

鸭瘟病毒（DPV）是一种主要的传染性和致命性疾病，可感染鹅、鸭和其他野生水禽。DPV 的 UL49 基因产物 VP22 是最丰富的包膜蛋白之一。然而，DPV VP22 的作用尚不清楚，需要进一步阐明。在本研究中，我们发现 UL49 基因缺失导致鸭胚成纤维细胞（DEF）中病毒生长曲线下降和斑块变小，证实 DPV VP22 是病毒在体外有效生长所必需的。此外，UL49 基因缺失抑制了病毒的二次包被、病毒粒子的释放以及病毒在细胞间的传播。我们的研究表明 VP22 对 DPV 的二次包被、释放、细胞间传播和病毒 RNA 的积累非常重要。这些发现为进一步研究 VP22 在 DPV 或其他疱疹病毒中的功能提供了基础。