College of Chemistry and Bioengineering, Guilin University of Technology, Guilin, 541004, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, 50 Zhongling Street, Nanjing, 210014, China.
Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, 50 Zhongling Street, Nanjing, 210014, China; Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, China; School of Food and Biological Engineering, Jiangsu University, 301 Zhenjiang City University Road, Zhenjiang, 212001, China.
Chemosphere. 2021 Mar;266:129020. doi: 10.1016/j.chemosphere.2020.129020. Epub 2020 Nov 19.
Root exudates are released by plant roots and are important carrier substances for material exchange and information transmission among plants and the rhizosphere. In the present study, the effect of dinotefuran on root exudates of Chinese cabbage (Brassica rapa var. chinensis) was investigated. The physiological activities revealed that dinotefuran uptake caused oxidative stress in vegetable tissues even at low dinotefuran exposure levels. The metabolic profile of plant root exudates acquired by LC-QTOF/MS was clearly changed by dinotefuran, where the numbers of both up- and down-regulated MS peaks increased with increasing dinotefuran concentration. Under dinotefuran stress, some osmotic adjustment substances (proline and betaine) and defence-related metabolites (spermidine, phenylalanine and some phenolic acids) were significantly upregulated, which may help plants adapt to adverse environmental conditions. Specifically, the contents of some phenylalanine-derived secondary metabolites increased with increasing dinotefuran concentration, which may increase the external detoxification ability of plants. Moreover, respiration metabolism was significantly affected, where some intermediates in the TCA cycle (succinic acid and malic acid) were upregulated with low-level dinotefuran exposure; however, anaerobic respiration products (lactic acid and 3-phenyllactic acid) were accumulated at high exposure levels. In addition, the release of glucosinolates was significantly inhibited in both dinotefuran treatment groups.
根系分泌物是由植物根系释放的，是植物与根际之间物质交换和信息传递的重要载体物质。本研究探讨了噻虫嗪对白菜（ Brassica rapa var. chinensis ）根系分泌物的影响。生理活性研究表明，即使在低浓度噻虫嗪暴露下，噻虫嗪的吸收也会导致蔬菜组织发生氧化应激。通过 LC-QTOF/MS 获得的植物根系分泌物代谢谱明显受到噻虫嗪的影响，随着噻虫嗪浓度的增加，上调和下调的 MS 峰数量都增加了。在噻虫嗪胁迫下，一些渗透调节物质（脯氨酸和甜菜碱）和防御相关代谢物（亚精胺、苯丙氨酸和一些酚酸）显著上调，这可能有助于植物适应不利的环境条件。具体来说，一些苯丙氨酸衍生的次生代谢物的含量随着噻虫嗪浓度的增加而增加，这可能增加了植物的外部解毒能力。此外，呼吸代谢受到显著影响，低浓度噻虫嗪暴露时，三羧酸循环（琥珀酸和苹果酸）的一些中间产物上调；然而，在高暴露水平下，积累了无氧呼吸产物（乳酸和 3-苯乳酸）。此外，在噻虫嗪处理组中，硫代葡萄糖苷的释放均受到显著抑制。
